Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 887110..887770 Replicon chromosome
Accession NZ_CP074448
Organism Enterobacter asburiae strain S3_CRE3

Toxin (Protein)


Gene name higB Uniprot ID A0A808IFD9
Locus tag KHV90_RS03990 Protein ID WP_048980127.1
Coordinates 887110..887463 (+) Length 118 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1Z3MX79
Locus tag KHV90_RS03995 Protein ID WP_029741460.1
Coordinates 887468..887770 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV90_RS03970 (KHV90_03960) 883252..884169 + 918 WP_033145933.1 nitrogen assimilation transcriptional regulator NAC -
KHV90_RS03975 (KHV90_03965) 884266..885216 + 951 WP_023336219.1 HTH-type transcriptional regulator Cbl -
KHV90_RS03980 (KHV90_03970) 885260..885865 - 606 WP_048980132.1 glutathione S-transferase family protein -
KHV90_RS03985 (KHV90_03975) 886039..886932 + 894 WP_048980130.1 LysR substrate-binding domain-containing protein -
KHV90_RS03990 (KHV90_03980) 887110..887463 + 354 WP_048980127.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KHV90_RS03995 (KHV90_03985) 887468..887770 + 303 WP_029741460.1 XRE family transcriptional regulator Antitoxin
KHV90_RS04000 (KHV90_03990) 887846..888766 - 921 WP_048980124.1 LysR family transcriptional regulator -
KHV90_RS04005 (KHV90_03995) 888908..889309 + 402 WP_048980122.1 RidA family protein -
KHV90_RS04015 (KHV90_04005) 889604..891793 - 2190 WP_045356712.1 TonB-dependent siderophore receptor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 875740..887770 12030


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-106)

Antitoxin

Cro/C1-type helix-turn-helix domain

(33-79)


Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13599.44 Da        Isoelectric Point: 8.9620

>T202187 WP_048980127.1 NZ_CP074448:887110-887463 [Enterobacter asburiae]
VWAINTTDRFDRWFTSLDDTDRASVLAALLVLREKGPGLSRPYADTLKGSRYSNMKELRIQSKGDPLRAFFAFDPNRTGI
VLCAGNKVGNERRFYDEMLLVADREFTHWLDRLKERE

Download         Length: 354 bp

>T202187 NZ_CP099338:c2715750-2715496 [Escherichia fergusonii]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACTCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11050.83 Da        Isoelectric Point: 5.7316

>AT202187 WP_029741460.1 NZ_CP074448:887468-887770 [Enterobacter asburiae]
MGRTLEQLIADEKPEVVASAQAIATDILLNIHLAELREKVQKTQVEMAQALGIRQPTVAVMEKPGRDLKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Download         Length: 303 bp

>AT202187 NZ_CP099338:c2715998-2715747 [Escherichia fergusonii]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAACGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCCCTGGAAGAGACGGCTTATC
TATTGCGTTCCCCCGCTAATGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A808IFD9

Antitoxin

Source ID Structure
AlphaFold DB A0A1Z3MX79

References