Detailed information of TA system TA202096

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4489913..4490667
• Replicon: chromosome
• Accession: NZ_CP074425
• Organism: Salmonella sp. SJTUF15034

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5RFE2
• Locus tag: KFD68_RS21715
• Protein ID: WP_000558168.1
• Coordinates: 4489913..4490224 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KFD68_RS21720
• Protein ID: WP_001259009.1
• Coordinates: 4490221..4490667 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFD68_RS21685 (4485571)	4485571..4486473	+	903	WP_000331364.1	formate dehydrogenase subunit beta	-
KFD68_RS21690 (4486470)	4486470..4487105	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
KFD68_RS21695 (4487102)	4487102..4488031	+	930	WP_000027736.1	formate dehydrogenase accessory protein FdhE	-
KFD68_RS21700 (4488078)	4488078..4488368	-	291	WP_000362050.1	DNA-binding transcriptional regulator	-
KFD68_RS21705 (4488369)	4488369..4488680	-	312	WP_001159635.1	cytotoxic translational repressor of toxin-antitoxin stability system	-
KFD68_RS21710 (4488898)	4488898..4489827	+	930	WP_001127705.1	alpha/beta hydrolase	-
KFD68_RS21715 (4489913)	4489913..4490224	+	312	WP_000558168.1	type II toxin-antitoxin system HigB family toxin	Toxin
KFD68_RS21720 (4490221)	4490221..4490667	+	447	WP_001259009.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
KFD68_RS21725 (4490682)	4490682..4491623	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
KFD68_RS21730 (4491668)	4491668..4492105	-	438	WP_000560968.1	D-aminoacyl-tRNA deacylase	-
KFD68_RS21735 (4492102)	4492102..4492974	-	873	WP_000921423.1	virulence factor BrkB family protein	-
KFD68_RS21740 (4492968)	4492968..4493567	-	600	WP_000965695.1	glucose-1-phosphatase	-
KFD68_RS21745 (4493758)	4493758..4494561	-	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
KFD68_RS21750 (4494595)	4494595..4495491	-	897	WP_001520529.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12324.26 Da
• Isoelectric Point: 9.3143

>T202096 WP_000558168.1 NZ_CP074425:4489913-4490224 [Salmonella sp. SJTUF15034]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTLTQPEEMKRYIPSLDNFKHRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYDRGNKE

Nucleotide

• Length: 312 bp

>T202096 NZ_CP099314:617988-618275 [Enterobacter hormaechei]
ATGCCTATAGAGTATGAATGGGATAGCAATAAGGCGAAAAGCAATCTGCAAAAACACGCCATTCGTTTTGAAGATGCAGT
TCTGGTTTTTGACGATCCCTGCCATTTGTCTGTTCAGGATCGTTATGAAAACGGGGAGTTTCGCTGGCAAACCATTGGTC
TTGTACAGGGCGTTATTGTCATTCTGGTCGCGCACACGGTACGTTTTGAAAGCGGGGATGAAATCATACGTATCATCAGT
GCGCGTAAAGCGGATCGTAAAGAGAGGAGGCGTTATGAGCATCGTTAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16720.06 Da
• Isoelectric Point: 6.6451

>AT202096 WP_001259009.1 NZ_CP074425:4490221-4490667 [Salmonella sp. SJTUF15034]
MRTHRQMDATSAKKIVDTFSDAVKSVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT202096 NZ_CP099314:618262-618564 [Enterobacter hormaechei]
ATGAGCATCGTTAAACATAAACGCGGTAGTGCACCGGAAGTGAGTGCGCGCCGGGAGGCTGAACTCAAAGCGCTGGCGGA
TAAACCCGACAGGGAAATTGATTACAGCGATATACCGGCCACGGAGGATGATCGCTGGGCCGGTGCCGTGCGGGGAAAAT
TCTATCGACCGCTCAAAACTCAGGCATCAGTACGCATTGACGCCGACGTGATGGAGTGGCTTAAACGGCCAGGGAAAGGT
TACCAGACGCGGCTTAATGCCATTTTGCGCGAGGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A1R2S541