Detailed information of TA system TA202064

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5983032..5983625
• Replicon: chromosome
• Accession: NZ_CP074412
• Organism: Pseudomonas viridiflava strain U625

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: KHW14_RS26425
• Protein ID: WP_122409317.1
• Coordinates: 5983347..5983625 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: KHW14_RS26420
• Protein ID: WP_025993256.1
• Coordinates: 5983032..5983337 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHW14_RS26390 (KHW14_26390)	5978744..5979436	+	693	WP_025993252.1	HAD-IA family hydrolase	-
KHW14_RS26395 (KHW14_26395)	5979515..5979844	-	330	WP_004887402.1	transcriptional regulator SutA	-
KHW14_RS26400 (KHW14_26400)	5979944..5980369	-	426	WP_025993253.1	secondary thiamine-phosphate synthase enzyme YjbQ	-
KHW14_RS26405 (KHW14_26405)	5980612..5981949	-	1338	WP_004887406.1	ammonium transporter	-
KHW14_RS26410 (KHW14_26410)	5981984..5982322	-	339	WP_002555808.1	P-II family nitrogen regulator	-
KHW14_RS26415 (KHW14_26415)	5982742..5983008	+	267	WP_004887407.1	accessory factor UbiK family protein	-
KHW14_RS26420 (KHW14_26420)	5983032..5983337	-	306	WP_025993256.1	HigA family addiction module antitoxin	Antitoxin
KHW14_RS26425 (KHW14_26425)	5983347..5983625	-	279	WP_122409317.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KHW14_RS26430 (KHW14_26430)	5983912..5984535	+	624	WP_043303441.1	hypothetical protein	-
KHW14_RS26435 (KHW14_26435)	5984566..5985873	-	1308	WP_122432618.1	type II toxin-antitoxin system HipA family toxin	-
KHW14_RS26440 (KHW14_26440)	5985863..5986186	-	324	WP_058430394.1	helix-turn-helix domain-containing protein	-
KHW14_RS26445 (KHW14_26445)	5986488..5987483	-	996	WP_277302688.1	MsnO8 family LLM class oxidoreductase	-
KHW14_RS26450 (KHW14_26450)	5987808..5988326	+	519	WP_122522433.1	Bro-N domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (10-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-92)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10416.01 Da
• Isoelectric Point: 8.5502

>T202064 WP_122409317.1 NZ_CP074412:c5983625-5983347 [Pseudomonas viridiflava]
MIVSFKCGDTRGLFQHGKTRLWPALKAVAERKLAMLDAAVLLSDLRSPPGNRLEILDGDRRGQHSIRINAQFRICFVWGE
NGPENVEIVDYH

Nucleotide

• Length: 279 bp

>T202064 NZ_CP099304:c5212660-5212373 [Klebsiella michiganensis]
ATGCCAATGGAGTTTGAATGGGATGCGAACAAGGCAATAAGTAATCTAAGAAAACATGGAATCCGGTTTGAAGAAGCGGT
GCTCGTCTTTGACGATCCCCGGCATCTTTCCCGACAGGATCGTTATGAGAATGGTGAATATCGCTGGCAAACTCTCGGCC
TCGTTCATGGGATCATTGTTATCATGGTTGCGCATTCGGTCCGTTTTGAAAGTGGAACAGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCGGACAGCAAAGAGAGAAATCGTTATGAACATGGTTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11245.07 Da
• Isoelectric Point: 8.5205

>AT202064 WP_025993256.1 NZ_CP074412:c5983337-5983032 [Pseudomonas viridiflava]
MTKNGMRPVHPGEVLKEEYLEPMGLTAAALARALNVSTPTVNDIVLQRRGVSADVALRLGVCLETSPEFWLNLQLTYDLR
KAEIEKGAQIRDQVRRLARCA

Nucleotide

• Length: 306 bp

>AT202064 NZ_CP099304:c5212386-5212084 [Klebsiella michiganensis]
ATGAACATGGTTAAACATAAACGTGACAATCCGGATACGCTTACGGCTAAGCGAGACGATGAGCTTAAAGCGCTGGCCAA
CAAAACGGATGATGAAATCGATTATAGCGATATTCCCGCCACAGACGACGCACAGTGGTCAGATGCCGTACGCGGTAAAT
TTTACCGACCGCTAAAAACTCAGGCTTCTGTCCGTATTGATGCCGATATTATGGAGTGGCTTAAACGTCCCGGGAAAGGT
TATCAAACGCGACTGAATGCGATCTTAAGAGAAGCGATGATTCGCGATCTTAACAAGAAATAG