Detailed information of TA system TA202048

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 45859..46128
• Replicon: plasmid p1-2AR
• Accession: NZ_CP074408
• Organism: Escherichia coli strain GDAR2-2

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KHW16_RS24125
• Protein ID: WP_001372321.1
• Coordinates: 46003..46128 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	45859..45924 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHW16_RS24085	40895..41326	-	432	Protein_47	hypothetical protein	-
KHW16_RS24090	41628..42155	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
KHW16_RS24095	42213..42446	+	234	WP_000006003.1	DUF905 family protein	-
KHW16_RS24100	42507..44471	+	1965	WP_000117316.1	ParB/RepB/Spo0J family partition protein	-
KHW16_RS24105	44540..44974	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
KHW16_RS24110	44971..45733	+	763	Protein_52	plasmid SOS inhibition protein A	-
-	45702..45926	+	225	NuclAT_0	-	-
-	45702..45926	+	225	NuclAT_0	-	-
-	45702..45926	+	225	NuclAT_0	-	-
-	45702..45926	+	225	NuclAT_0	-	-
KHW16_RS24115	45711..45890	-	180	WP_001309233.1	hypothetical protein	-
-	45859..45924	-	66	-	-	Antitoxin
KHW16_RS24120	45912..46061	+	150	Protein_54	plasmid maintenance protein Mok	-
KHW16_RS24125	46003..46128	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
KHW16_RS24130	46447..46744	-	298	Protein_56	hypothetical protein	-
KHW16_RS24135	47044..47340	+	297	WP_001272251.1	hypothetical protein	-
KHW16_RS24140	47451..48272	+	822	WP_001234445.1	DUF932 domain-containing protein	-
KHW16_RS24145	48569..49171	-	603	Protein_59	transglycosylase SLT domain-containing protein	-
KHW16_RS24150	49492..49875	+	384	WP_001151538.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KHW16_RS24155	50062..50751	+	690	WP_016239105.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..71810	71810

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T202048 WP_001372321.1 NZ_CP074408:46003-46128 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T202048 NZ_CP099303:276958-277326 [Citrobacter freundii]
ATGACGCTACAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTGACGCCTGGTGTCGCAGG
TATGCCTGACCCCGGTCGTGCGGAAGCGCTGATGTATCGGGTATTGAATCAAATCGAATATGAAGGTGTGACTGACGTGT
GGCTACTGGCGGCAATGCATTTGTTAGCCATTTCCCGTGGGCATATCTTTAATGATGGTAACAAGCGTACTGCGTTATTT
ATTACGTTGCTGTTTTTAAAACGTAATGGGATCGTATTATCTGCAAACCCTGATTTTGTCGCCATGACGGTTGAAGCGGC
AGCAGGACAACTCACGTTAGAACAAATTGCGCATCGTCTGCGCCATTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT202048 NZ_CP074408:c45924-45859 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin