Detailed information of TA system TA202045

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 14453..14706
• Replicon: plasmid p1-2AR
• Accession: NZ_CP074408
• Organism: Escherichia coli strain GDAR2-2

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: KHW16_RS23910
• Protein ID: WP_001336447.1
• Coordinates: 14557..14706 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	14453..14509 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHW16_RS23880 (10365)	10365..11111	+	747	WP_019842127.1	conjugal transfer pilus acetylase TraX	-
KHW16_RS23885 (11166)	11166..11723	+	558	WP_000139329.1	fertility inhibition protein FinO	-
KHW16_RS23890 (11875)	11875..12078	+	204	WP_001336517.1	hypothetical protein	-
KHW16_RS23895 (12820)	12820..13281	+	462	WP_019842128.1	thermonuclease family protein	-
KHW16_RS23900 (13550)	13550..13768	+	219	WP_023150739.1	hypothetical protein	-
KHW16_RS23905 (13921)	13921..14358	+	438	WP_000872609.1	hypothetical protein	-
- (14453)	14453..14509	-	57	NuclAT_1	-	Antitoxin
- (14453)	14453..14509	-	57	NuclAT_1	-	Antitoxin
- (14453)	14453..14509	-	57	NuclAT_1	-	Antitoxin
- (14453)	14453..14509	-	57	NuclAT_1	-	Antitoxin
KHW16_RS23910 (14557)	14557..14706	+	150	WP_001336447.1	Hok/Gef family protein	Toxin
KHW16_RS23915 (14991)	14991..15248	+	258	WP_000084404.1	replication regulatory protein RepA	-
KHW16_RS23920 (15484)	15484..15558	+	75	WP_001365705.1	RepA leader peptide Tap	-
KHW16_RS23925 (15551)	15551..16408	+	858	WP_000130646.1	incFII family plasmid replication initiator RepA	-
KHW16_RS23930 (17321)	17321..17458	+	138	Protein_16	XRE family transcriptional regulator	-
KHW16_RS23935 (17478)	17478..17741	+	264	WP_001089473.1	hypothetical protein	-
KHW16_RS23940 (17731)	17731..18030	+	300	WP_001528606.1	type II toxin-antitoxin system RelE/ParE family toxin	-
KHW16_RS23945 (18066)	18066..18731	-	666	WP_001535733.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..71810	71810

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T202045 WP_001336447.1 NZ_CP074408:14557-14706 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T202045 NZ_CP099298:c4775783-4775424 [Citrobacter freundii]
ATGACGAAACCACTTTACTGGGTTGGGCAGGCCAGAAAAGACTTGCTTGCAATGCCAGAACACGTTCGGGATACGTTTGG
GTTTGCTCTTTGGCTTGCGCAGCAGGGTAAACAGCATTCACAGACTAAACCATTAAAAGGATTTGGTGGAGCAGGGGTTC
TGGAGGTCGTTGAGGATTACCACGGTAGCGCCTGGCGGGCGGTTTACACCATCCAGTTGAAAAATGCGGTTTATGTTCTC
CATGTTTTTCAGAAGAAATCCGTTTCCGGGAAGGCAACCCCGAAACCTGAAATTGACCTGATTTATCAGCGGCTCAAGGC
TGCACAGCGCCATGCGCAGGAGAGTGGATATGTCATATGA

Antitoxin

Nucleotide

• Length: 57 bp

>AT202045 NZ_CP074408:c14509-14453 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin