Detailed information of TA system TA202032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 3180523..3181025
• Replicon: chromosome
• Accession: NZ_CP074407
• Organism: Escherichia coli strain GDAR2-2

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: KHW16_RS15285
• Protein ID: WP_238557792.1
• Coordinates: 3180771..3181025 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: KHW16_RS15280
• Protein ID: WP_001259255.1
• Coordinates: 3180523..3180774 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHW16_RS15255 (3175701)	3175701..3176768	-	1068	WP_000080087.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
KHW16_RS15260 (3176768)	3176768..3177838	-	1071	WP_001297360.1	histidinol-phosphate transaminase	-
KHW16_RS15265 (3177835)	3177835..3179139	-	1305	WP_033873485.1	histidinol dehydrogenase	-
KHW16_RS15270 (3179145)	3179145..3180044	-	900	WP_000131756.1	ATP phosphoribosyltransferase	-
KHW16_RS15275 (3180190)	3180190..3180240	-	51	WP_001364200.1	his operon leader peptide	-
KHW16_RS15280 (3180523)	3180523..3180774	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
KHW16_RS15285 (3180771)	3180771..3181025	+	255	WP_238557792.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
KHW16_RS15290 (3181108)	3181108..3181932	+	825	WP_000754758.1	SDR family oxidoreductase	-
KHW16_RS15295 (3181978)	3181978..3182907	+	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
KHW16_RS15300 (3183122)	3183122..3183184	+	63	WP_010723108.1	membrane protein YoeI	-
KHW16_RS15305 (3183174)	3183174..3184532	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
KHW16_RS15310 (3184711)	3184711..3185769	+	1059	WP_023568741.1	thiosulfate utilization transporter TsuA/YeeE	-
KHW16_RS15315 (3185783)	3185783..3186010	+	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10140.56 Da
• Isoelectric Point: 7.2749

>T202032 WP_238557792.1 NZ_CP074407:3180771-3181025 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
LYHY

Nucleotide

• Length: 255 bp

>T202032 NZ_CP099291:c4839859-4839485 [Citrobacter freundii]
ATGGTCAAAGGATCCGCGCTTTTTGATACCAACATCCTCATCGATCTGTTTAGTGGGAGAAATGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAATGCAATCAGCCTCATTACATGGATGGAGGTGATGGTGGGTGCAAAAAAATACCATCAGG
AAGCCCGTACTCGTGTTGCGCTAGGTGCTTTTAACGTGATCGGCGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGACAGGAATATGGGATGAAATTGCCGGATGCCATTATTTTGGCAACAGCACAGATTCATCGTCTTACGCTGGTTACGCG
AAATACTAAGGATTTTGCAGGCCTATCCGGTGTGGTCACACCGTATACATTATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT202032 WP_001259255.1 NZ_CP074407:3180523-3180774 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT202032 NZ_CP099291:c4840101-4839859 [Citrobacter freundii]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGAAGTGATTAAACGTCTTGATGATCTCAAGGT
GCAGCGCAACCTGCCGCGAGCAGAACTTTTACGGGAGGCCGTTGAACAGTATCTTGAGAGACAGGATCAGGCTGAAACCA
CAATTTCACGTGCTCTTGGGCTGTGGCAAGGCTGTGAGGAAGATGGTGTTGAATACCAGCGTAAACTCCGTGAGGAATGG
TAA

Structures

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0