Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1886090..1886889 Replicon chromosome
Accession NZ_CP074407
Organism Escherichia coli strain GDAR2-2

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag KHW16_RS08890 Protein ID WP_000347267.1
Coordinates 1886090..1886554 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KHW16_RS08895 Protein ID WP_001307405.1
Coordinates 1886554..1886889 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHW16_RS08860 (1881091) 1881091..1881525 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KHW16_RS08865 (1881543) 1881543..1882421 - 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
KHW16_RS08870 (1882411) 1882411..1883190 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KHW16_RS08875 (1883201) 1883201..1883674 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KHW16_RS08880 (1883697) 1883697..1884977 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
KHW16_RS08885 (1885226) 1885226..1886035 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KHW16_RS08890 (1886090) 1886090..1886554 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KHW16_RS08895 (1886554) 1886554..1886889 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KHW16_RS08900 (1887038) 1887038..1888609 - 1572 WP_001273741.1 galactarate dehydratase -
KHW16_RS08905 (1888984) 1888984..1890318 + 1335 WP_087889555.1 galactarate/glucarate/glycerate transporter GarP -
KHW16_RS08910 (1890334) 1890334..1891104 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T202028 WP_000347267.1 NZ_CP074407:c1886554-1886090 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T202028 NZ_CP099291:3982256-3982597 [Citrobacter freundii]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATAGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGTAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT202028 WP_001307405.1 NZ_CP074407:c1886889-1886554 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT202028 NZ_CP099291:3981918-3982235 [Citrobacter freundii]
ATGAGCAAAATCACATGGGGTCTGCAGCGTGATATCACGCCACGTCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATGACCGGCAAGTTCAGTGACATCGAATACCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACTGGTGAACTCAGCACCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References