Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 1086774..1087376 Replicon chromosome
Accession NZ_CP074407
Organism Escherichia coli strain GDAR2-2

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag KHW16_RS05025 Protein ID WP_000897305.1
Coordinates 1087065..1087376 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KHW16_RS05020 Protein ID WP_000356397.1
Coordinates 1086774..1087064 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHW16_RS04995 (1082699) 1082699..1083601 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
KHW16_RS05000 (1083598) 1083598..1084233 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
KHW16_RS05005 (1084230) 1084230..1085159 + 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
KHW16_RS05010 (1085489) 1085489..1085731 - 243 WP_001087409.1 protein YiiF -
KHW16_RS05015 (1085951) 1085951..1086169 - 219 WP_001315930.1 CopG family transcriptional regulator -
KHW16_RS05020 (1086774) 1086774..1087064 - 291 WP_000356397.1 NadS family protein Antitoxin
KHW16_RS05025 (1087065) 1087065..1087376 - 312 WP_000897305.1 hypothetical protein Toxin
KHW16_RS05030 (1087605) 1087605..1088513 + 909 WP_021293166.1 alpha/beta hydrolase -
KHW16_RS05035 (1088577) 1088577..1089518 - 942 WP_001343389.1 fatty acid biosynthesis protein FabY -
KHW16_RS05040 (1089563) 1089563..1090000 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
KHW16_RS05045 (1089997) 1089997..1090869 - 873 WP_000920762.1 virulence factor BrkB family protein -
KHW16_RS05050 (1090863) 1090863..1091462 - 600 WP_001295269.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T202026 WP_000897305.1 NZ_CP074407:c1087376-1087065 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T202026 NZ_CP099291:2157617-2157720 [Citrobacter freundii]
GGCAGGGTAACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT202026 WP_000356397.1 NZ_CP074407:c1087064-1086774 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT202026 NZ_CP099291:c2157725-2157582 [Citrobacter freundii]
CACATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTAAGTTACCCTGCCATTTAAGAATAGATGACAGCGCCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References