Detailed information of TA system TA202001

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3007835..3008425
• Replicon: chromosome
• Accession: NZ_CP074376
• Organism: Mycolicibacterium neoaurum strain MN2019

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: MN2019_RS14235
• Protein ID: WP_213446915.1
• Coordinates: 3007835..3008116 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MN2019_RS14240
• Protein ID: WP_213446916.1
• Coordinates: 3008129..3008425 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MN2019_RS14230 (MN2019_14230)	3004920..3007523	-	2604	WP_213446914.1	DUF4236 domain-containing protein	-
MN2019_RS14235 (MN2019_14235)	3007835..3008116	+	282	WP_213446915.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MN2019_RS14240 (MN2019_14240)	3008129..3008425	+	297	WP_213446916.1	HigA family addiction module antitoxin	Antitoxin
MN2019_RS14245 (MN2019_14245)	3008621..3009391	+	771	WP_213446917.1	SDR family NAD(P)-dependent oxidoreductase	-
MN2019_RS14250 (MN2019_14250)	3009616..3010047	+	432	WP_213446918.1	MmpS family transport accessory protein	-
MN2019_RS14255 (MN2019_14255)	3010044..3012923	+	2880	WP_213446919.1	RND family transporter	-
MN2019_RS14260 (MN2019_14260)	3012932..3013276	+	345	WP_213446920.1	DUF732 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (5-81)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10811.23 Da
• Isoelectric Point: 9.1427

>T202001 WP_213446915.1 NZ_CP074376:3007835-3008116 [Mycolicibacterium neoaurum]
VIRSFRDGDTQKVWELRRPKGISVELAKAARRKMQILDAAENINDLRVPPGNRLEKLQPPREGQHSIRVNDQFRICFTWR
DGGADDVELTDYH

Nucleotide

• Length: 282 bp

>T202001 NZ_CP099254:932716-933114 [Citrobacter freundii]
GTGGTCCAGTGGCAATCTGATTTACGCGTCTCCTGGCGCGCACAGTGGATTTCGCTGCTCATTCATGGGCTAGTGGCGGT
GCTCATTTTATTGATGCCATGGCCACTGAGTTACACCCCATTATGGTTGATTTTGCTCTCTCTGGTGGTGTTCGATTGCG
TGCGCAGTCAGCGCCGTATTAACGCTTGTCAGGGAGAGATTAGACTATTAATGGATGGCCGCCTGCGTTGGCAAGGGCAA
GAGTGGACGATTGTTAGCGCACCCTGGATGATAAAAACGGGCATGATGTTGCGTTTGCGGTCAAGTACAGGAAAACGTCA
GCATCTGTGGCTGGCGGCGGACAGTATGGATGATGCCGAGTGGCGGGATTTGCGTCGGTTGATATTACAGCAGAAATAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10873.43 Da
• Isoelectric Point: 5.6993

>AT202001 WP_213446916.1 NZ_CP074376:3008129-3008425 [Mycolicibacterium neoaurum]
MADFAPTHPGEILAEEFLVPMGISAYRLAQEIHVPQTRLSEIIRGRRAITADTGLRLSRALGLSDMFWINLQARYDADVA
RAERGDELDRIRPLAAAG

Nucleotide

• Length: 297 bp

>AT202001 NZ_CP099254:932469-932735 [Citrobacter freundii]
ATGGATATTAACAACAAAGCACGTATTCATTGGGCATGCCGTCGCGGTATGCGTGAGCTGGACATTTCGATCATGCCGTT
TTTCGAACATGAGTATGACAACTTAAGTGATGACGAAAAACGCATCTTTATCCGTTTGCTGGAATGTGATGACCCCGATT
TATTTAACTGGTTGATGAATCACGGGAAGCCGGCTGATGAGCAGCTGGAGCAAATGGTACGGCTTATCCAGACACGGAAT
CGGGAACGTGGTCCAGTGGCAATCTGA