Detailed information of TA system TA201988

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4649371..4649973
• Replicon: chromosome
• Accession: NZ_CP074366
• Organism: Escherichia coli strain ZYB62

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: KG092_RS22370
• Protein ID: WP_000897305.1
• Coordinates: 4649662..4649973 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KG092_RS22365
• Protein ID: WP_000356397.1
• Coordinates: 4649371..4649661 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KG092_RS22340 (4645296)	4645296..4646198	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
KG092_RS22345 (4646195)	4646195..4646830	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
KG092_RS22350 (4646827)	4646827..4647756	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
KG092_RS22355 (4648086)	4648086..4648328	-	243	WP_001087409.1	protein YiiF	-
KG092_RS22360 (4648548)	4648548..4648766	-	219	WP_001315930.1	CopG family transcriptional regulator	-
KG092_RS22365 (4649371)	4649371..4649661	-	291	WP_000356397.1	NadS family protein	Antitoxin
KG092_RS22370 (4649662)	4649662..4649973	-	312	WP_000897305.1	hypothetical protein	Toxin
KG092_RS22375 (4650202)	4650202..4651110	+	909	WP_001162704.1	alpha/beta hydrolase	-
KG092_RS22380 (4651174)	4651174..4652115	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
KG092_RS22385 (4652160)	4652160..4652597	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
KG092_RS22390 (4652594)	4652594..4653466	-	873	WP_000920762.1	virulence factor BrkB family protein	-
KG092_RS22395 (4653460)	4653460..4654059	-	600	WP_001295269.1	glucose-1-phosphatase	-
KG092_RS22400 (4654158)	4654158..4654943	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T201988 WP_000897305.1 NZ_CP074366:c4649973-4649662 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T201988 NZ_CP099252:261789-262064 [Klebsiella aerogenes]
ATGGAGCAGCAGGTTTTATCTTTACGTAAAAAACAGCGCAACACGCTGGAGCAGCTGTTTAAAACCCCCGTGCCGCAGGG
TATTAAATGGGCCGACATTGAATCGCTAATTAAAGCGTTAGGTGGGGAGATTAAAGAAGGACGCGGCTCGCGCTGTAAAT
TTTTGTTGAACCACAGCATTGCCAGCTTTCACAGGCCTCACCCTTCGCCGGATACTGACAAAGGCGCCGTGGAGAGCGTG
CGCGATTGGTTAATCACTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT201988 WP_000356397.1 NZ_CP074366:c4649661-4649371 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT201988 NZ_CP099252:262061-262420 [Klebsiella aerogenes]
ATGATCAAACCTAAAACGCCAAATTCAATGGAGATTGCCGGGCAGCCTGCTGTTATTAACTACGTGCCTGAACTCAATGC
ATTCCGCGGTAAGTTCCTGGGATTATCAGGATATTGCGATTTTGTTTCCGATAGCATTCAAGGGTTACAACAGGAGGGCG
AGATATCATTACAGGAATATCTTGCTGATTGTCGTGAAGCGGGCATCGAGCCTTATGCTCAGCCGGAAAAGCTGAAAACC
TTCACATTGCGCTATCCTGAATCATTTGGTGAGCGTCTTAGCTCCGCCGCTGCAGAGGAGCAGGTTTCCGTAAATACCTG
GATCCTTGAGACGCTCAATGAACGTCTCAAACAGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7