Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | DinJ-YafQ (relBE)/YafQ-RelB |
| Location | 3820927..3821468 | Replicon | chromosome |
| Accession | NZ_CP074366 | ||
| Organism | Escherichia coli strain ZYB62 | ||
Toxin (Protein)
| Gene name | yafQ | Uniprot ID | U9XPG0 |
| Locus tag | KG092_RS18500 | Protein ID | WP_000615976.1 |
| Coordinates | 3821190..3821468 (+) | Length | 93 a.a. |
Antitoxin (Protein)
| Gene name | dinJ | Uniprot ID | S1EWQ0 |
| Locus tag | KG092_RS18495 | Protein ID | WP_000729704.1 |
| Coordinates | 3820927..3821187 (+) | Length | 87 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KG092_RS18470 (3815963) | 3815963..3817102 | + | 1140 | WP_000785662.1 | flagellar type III secretion system protein FlhB | - |
| KG092_RS18475 (3817086) | 3817086..3819179 | + | 2094 | WP_001140827.1 | flagellar biosynthesis protein FlhA | - |
| KG092_RS18480 (3819284) | 3819284..3819562 | + | 279 | WP_000598760.1 | type II toxin-antitoxin system HicA family toxin | - |
| KG092_RS18485 (3819555) | 3819555..3819911 | + | 357 | WP_001030483.1 | type II toxin-antitoxin system HicB family antitoxin | - |
| KG092_RS18490 (3819968) | 3819968..3820741 | - | 774 | WP_000543895.1 | C40 family peptidase | - |
| KG092_RS18495 (3820927) | 3820927..3821187 | + | 261 | WP_000729704.1 | type II toxin-antitoxin system antitoxin DinJ | Antitoxin |
| KG092_RS18500 (3821190) | 3821190..3821468 | + | 279 | WP_000615976.1 | type II toxin-antitoxin system mRNA interferase toxin YafQ | Toxin |
| KG092_RS18505 (3821624) | 3821624..3822364 | + | 741 | WP_001225679.1 | peptidoglycan meso-diaminopimelic acid protein amidase | - |
| KG092_RS18510 (3822335) | 3822335..3823102 | - | 768 | WP_000333380.1 | class II glutamine amidotransferase | - |
| KG092_RS18515 (3823308) | 3823308..3823886 | - | 579 | WP_000284050.1 | D-sedoheptulose 7-phosphate isomerase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | gmhA/lpcA / rhs/PAAR / rhs/PAAR / tssF / tssA / hcp1/tssD1 | 3819555..3852954 | 33399 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 93 a.a. Molecular weight: 10800.59 Da Isoelectric Point: 10.0702
>T201986 WP_000615976.1 NZ_CP074366:3821190-3821468 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG
Download Length: 279 bp
>T201986 NZ_CP099223:c52341-51811 [Citrobacter freundii]
ATGGAGGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGGCAGCATAACAGTAAGATCTTACGTGGTTATGTTCTCGTGACGAGAGAGG
ATAAACCGCGTGTGATGGGGTACTACACGCTATCAGGAAGTTGCTTTGAGAAAATACTTCTACCCAGCAAAACACAACAA
AAGCGTGTACCCTACAAGAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCATCACCAGGGATA
TGGAGAAATACTTGTGACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGCATACACGGAATGTTCGTGG
AGGCGCTGAACGACAACGCCCAAAAATTCTACCTTCGACTGGGTTTTATACAGTTAAAGGAAGAGAACTGTAATTCGCTC
TTCTACCCGACAAAATCTATTGAAGAGTTGTTCGAAGTAAAAGATGAATAA
ATGGAGGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGGCAGCATAACAGTAAGATCTTACGTGGTTATGTTCTCGTGACGAGAGAGG
ATAAACCGCGTGTGATGGGGTACTACACGCTATCAGGAAGTTGCTTTGAGAAAATACTTCTACCCAGCAAAACACAACAA
AAGCGTGTACCCTACAAGAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCATCACCAGGGATA
TGGAGAAATACTTGTGACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGCATACACGGAATGTTCGTGG
AGGCGCTGAACGACAACGCCCAAAAATTCTACCTTCGACTGGGTTTTATACAGTTAAAGGAAGAGAACTGTAATTCGCTC
TTCTACCCGACAAAATCTATTGAAGAGTTGTTCGAAGTAAAAGATGAATAA
Antitoxin
Download Length: 87 a.a. Molecular weight: 9391.77 Da Isoelectric Point: 5.0758
>AT201986 WP_000729704.1 NZ_CP074366:3820927-3821187 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI
Download Length: 261 bp
>AT201986 NZ_CP099223:c52614-52345 [Citrobacter freundii]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCCTGGAACCTGGTTATGGATGCAATCATTAATCCGCCAGCACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCCTGGAACCTGGTTATGGATGCAATCATTAATCCGCCAGCACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | U9XPG0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| PDB | 4ML0 | |
| AlphaFold DB | A0A0E0Y6Z6 |