Detailed information of TA system TA201960

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 3496630..3497081
• Replicon: chromosome
• Accession: NZ_CP074365
• Organism: Xanthomonas translucens strain CFBP 8304

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KCU57_RS14970
• Protein ID: WP_230939940.1
• Coordinates: 3496932..3497081 (-)
• Length: 50 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KCU57_RS14965
• Protein ID: WP_237649421.1
• Coordinates: 3496630..3496812 (-)
• Length: 61 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KCU57_RS14945 (KCU57_14850)	3491985..3492488	+	504	WP_237649419.1	hypothetical protein	-
KCU57_RS14950 (KCU57_14855)	3492571..3493536	+	966	WP_065898826.1	IS1595 family transposase	-
KCU57_RS14955 (KCU57_14860)	3493727..3495505	+	1779	WP_237649420.1	DEAD/DEAH box helicase	-
KCU57_RS20075	3495661..3495783	-	123	WP_272932555.1	hypothetical protein	-
KCU57_RS14960 (KCU57_14865)	3495860..3496664	+	805	WP_237650971.1	IS5 family transposase	-
KCU57_RS14965 (KCU57_14870)	3496630..3496812	-	183	WP_237649421.1	hypothetical protein	Antitoxin
KCU57_RS14970	3496932..3497081	-	150	WP_230939940.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KCU57_RS14975 (KCU57_14880)	3497251..3497694	-	444	WP_237649422.1	nuclear transport factor 2 family protein	-
KCU57_RS14980 (KCU57_14885)	3497818..3498540	+	723	WP_237649423.1	helix-turn-helix transcriptional regulator	-
KCU57_RS14985 (KCU57_14890)	3498537..3499070	-	534	Protein_2962	endonuclease	-
KCU57_RS14990 (KCU57_14895)	3499179..3499430	+	252	WP_003468816.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
KCU57_RS14995 (KCU57_14900)	3499427..3499684	+	258	WP_237649424.1	Txe/YoeB family addiction module toxin	-
KCU57_RS15000 (KCU57_14905)	3499732..3500025	+	294	WP_237649425.1	hypothetical protein	-
KCU57_RS15005 (KCU57_14910)	3500153..3501520	-	1368	WP_237649185.1	IS5 family transposase	-
KCU57_RS15010 (KCU57_14915)	3501666..3501959	+	294	WP_237649426.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3490344..3505073	14729
	-	flank	IS/Tn	-	-	3492571..3493536	965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5326.12 Da
• Isoelectric Point: 8.5858

>T201960 WP_230939940.1 NZ_CP074365:c3497081-3496932 [Xanthomonas translucens]
MDDETYAKLQVLLQENLEVGDLIEGTGGLRKVRVAAKGHGKRGGARVIY

Nucleotide

• Length: 150 bp

>T201960 NZ_CP099140:c4668508-4668224 [Klebsiella pneumoniae]
ATGACCTATGAACTGGCGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 61 a.a.
• Molecular weight: 6755.79 Da
• Isoelectric Point: 8.9195

>AT201960 WP_237649421.1 NZ_CP074365:c3496812-3496630 [Xanthomonas translucens]
MSKFFEALMESVQQMDEIVRGERAPSREFHVDAIQVKQVRLPASPSLRAANKTLEVAVGR

Nucleotide

• Length: 183 bp

>AT201960 NZ_CP099140:c4668740-4668498 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA