201943

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3608720..3609261 Replicon chromosome
Accession NZ_CP074359
Organism Escherichia coli strain XJ6.2

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag KFQ22_RS17590 Protein ID WP_000615983.1
Coordinates 3608983..3609261 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag KFQ22_RS17585 Protein ID WP_000729703.1
Coordinates 3608720..3608980 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFQ22_RS17565 (3604395) 3604395..3605180 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
KFQ22_RS17570 (3605152) 3605152..3606864 + 1713 Protein_3434 flagellar biosynthesis protein FlhA -
KFQ22_RS17575 (3607088) 3607088..3607585 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
KFQ22_RS17580 (3607761) 3607761..3608510 - 750 WP_000056849.1 C40 family peptidase -
KFQ22_RS17585 (3608720) 3608720..3608980 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KFQ22_RS17590 (3608983) 3608983..3609261 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KFQ22_RS17595 (3609417) 3609417..3610157 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KFQ22_RS17600 (3610128) 3610128..3610895 - 768 WP_000333380.1 class II glutamine amidotransferase -
KFQ22_RS17605 (3611101) 3611101..3611679 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T201943 WP_000615983.1 NZ_CP074359:3608983-3609261 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T201943 NZ_CP099128:c334618-334301 [Citrobacter freundii]
ATGTTCACATTTATTGAGCTACAAGGATTTAGTAAACGCCGTCCGTTACTTTTGCCTGACGATGAGTTTCGCGCTTTTCA
AGAGGCGCTTATAGAGAACCCTGAGGCTGGAGATACTATCGCTGGTACAGGTGGATTCAGAAAAATTCGCTGGAGTCGCT
CTGGTATGGGCAAACGCAGCGGTATTAGGGTTATCTATTACAACGTGACGCGCAAAGGTCGAATCTATCTCGCTTTGCTC
TATCCTAAAAATGAACAGGATGATTTAACCGAAGAGCAAAAAAGAGTTTTAATGCACTTATCCAATATGCTGATTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT201943 WP_000729703.1 NZ_CP074359:3608720-3608980 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT201943 NZ_CP099128:c334248-333952 [Citrobacter freundii]
ATGAAAGACGAATTATTTGCCGATTTGCTGGCCAGTGCAGAAGAAATGGTGCGTATAGAGAAAGGTGAAGAAACACCTAA
GCCTGAACATGTACATACCTTTAGCGAAATTGATGTTAAAGCCATCCGCGAAGCAACAGGGTTACGCCAACAGGATTTTG
CCATAGCTGTTGGCGTGAGTTATGACCTGGTTAAAAGCTGGGAAACGAAACGACGCCAGCCGACTGGTGCACCAAGAAAA
CTGCTTTTATTAATTCAAAAAAATCCATTTATAATTAATCAATTAAAAGCAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
AlphaFold DB A0A5F1F716

References