Detailed information of TA system TA201942

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3602523..3603217
• Replicon: chromosome
• Accession: NZ_CP074359
• Organism: Escherichia coli strain XJ6.2

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: KFQ22_RS17550
• Protein ID: WP_001263489.1
• Coordinates: 3602523..3602921 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: KFQ22_RS17555
• Protein ID: WP_000554758.1
• Coordinates: 3602924..3603217 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3598111)	3598111..3598191	-	81	NuclAT_11	-	-
- (3598111)	3598111..3598191	-	81	NuclAT_11	-	-
- (3598111)	3598111..3598191	-	81	NuclAT_11	-	-
- (3598111)	3598111..3598191	-	81	NuclAT_11	-	-
KFQ22_RS17525 (3598787)	3598787..3599245	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
KFQ22_RS17530 (3599506)	3599506..3600963	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
KFQ22_RS17535 (3601020)	3601020..3601541	-	522	Protein_3427	peptide chain release factor H	-
KFQ22_RS17540 (3601537)	3601537..3601743	-	207	Protein_3428	RtcB family protein	-
KFQ22_RS17545 (3602061)	3602061..3602513	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
KFQ22_RS17550 (3602523)	3602523..3602921	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
KFQ22_RS17555 (3602924)	3602924..3603217	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
KFQ22_RS17560 (3603269)	3603269..3604324	-	1056	WP_001226164.1	DNA polymerase IV	-
KFQ22_RS17565 (3604395)	3604395..3605180	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
KFQ22_RS17570 (3605152)	3605152..3606864	+	1713	Protein_3434	flagellar biosynthesis protein FlhA	-
KFQ22_RS17575 (3607088)	3607088..3607585	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T201942 WP_001263489.1 NZ_CP074359:c3602921-3602523 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T201942 NZ_CP099128:276484-276852 [Citrobacter freundii]
ATGACGCTACAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTGACGCCTGGTGTCGCAGG
TATGCCTGACCCCGGTCGTGCGGAAGCGCTGATGTATCGGGTATTGAATCAAATCGAATATGAAGGTGTGACTGACGTGT
GGCTACTGGCGGCAATGCATTTGTTAGCCATTTCCCGTGGGCATATCTTTAATGATGGTAACAAGCGTACTGCGTTATTT
ATTACGTTGCTGTTTTTAAAACGTAATGGGATCGTATTATCTGCAAACCCTGATTTTGTCGCCATGACGGTTGAAGCGGC
AGCAGGACAACTCACGTTAGAACAAATTGCGCATCGTCTGCGCCATTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT201942 WP_000554758.1 NZ_CP074359:c3603217-3602924 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT201942 NZ_CP099128:276266-276487 [Citrobacter freundii]
ATGCGCACTATGAACTATAGTGAAGCTCGACAAAACCTGGCTGAAGTGCTGGAGAGCGCTGTCACTGGTGTGCCTGTTAC
CATTACGCGCCGTGGTCATAAACCTGCGGTAATCATCAGCGCGGAAGAGTTTGAACGCTATCAGGCCGCCAGACTTGATG
ATGAATTTGCCGACATAATGATGATTCATGGTAATGAAATCAGGGAGCTGGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3