201923

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 604041..604840 Replicon chromosome
Accession NZ_CP074359
Organism Escherichia coli strain XJ6.2

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag KFQ22_RS02960 Protein ID WP_000347275.1
Coordinates 604041..604505 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KFQ22_RS02965 Protein ID WP_001307405.1
Coordinates 604505..604840 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFQ22_RS02930 (599042) 599042..599476 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KFQ22_RS02935 (599494) 599494..600372 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KFQ22_RS02940 (600362) 600362..601141 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
KFQ22_RS02945 (601152) 601152..601625 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
KFQ22_RS02950 (601648) 601648..602928 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
KFQ22_RS02955 (603177) 603177..603986 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KFQ22_RS02960 (604041) 604041..604505 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KFQ22_RS02965 (604505) 604505..604840 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KFQ22_RS02970 (604989) 604989..606560 - 1572 WP_001273752.1 galactarate dehydratase -
KFQ22_RS02975 (606935) 606935..608269 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KFQ22_RS02980 (608285) 608285..609055 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T201923 WP_000347275.1 NZ_CP074359:c604505-604041 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T201923 NZ_CP099037:2103167-2103270 [Citrobacter freundii]
GGCAGGGTAACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT201923 WP_001307405.1 NZ_CP074359:c604840-604505 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT201923 NZ_CP099037:c2103275-2103132 [Citrobacter freundii]
CACATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTAAGTTACCCTGCCATTTAAGAATAGATGACAGCGCCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References