Detailed information of TA system TA201920

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 90618..90872
• Replicon: plasmid unnamed2
• Accession: NZ_CP074358
• Organism: Escherichia coli strain XJ6.1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: KFQ18_RS25155
• Protein ID: WP_001312851.1
• Coordinates: 90723..90872 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	90618..90679 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFQ18_RS25130 (87792)	87792..88538	+	747	Protein_101	conjugal transfer pilus acetylase TraX	-
KFQ18_RS25135 (88593)	88593..89153	+	561	WP_001523482.1	fertility inhibition protein FinO	-
KFQ18_RS25140 (89288)	89288..89500	+	213	WP_063079259.1	hypothetical protein	-
KFQ18_RS25145 (89744)	89744..89830	+	87	Protein_104	endonuclease	-
KFQ18_RS25150 (90011)	90011..90322	-	312	WP_000802277.1	hypothetical protein	-
- (90618)	90618..90679	-	62	NuclAT_1	-	Antitoxin
- (90618)	90618..90679	-	62	NuclAT_1	-	Antitoxin
- (90618)	90618..90679	-	62	NuclAT_1	-	Antitoxin
- (90618)	90618..90679	-	62	NuclAT_1	-	Antitoxin
KFQ18_RS25155 (90723)	90723..90872	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
KFQ18_RS25160 (91156)	91156..91413	+	258	WP_000083845.1	replication regulatory protein RepA	-
KFQ18_RS25165 (91647)	91647..91721	+	75	WP_001375168.1	RepA leader peptide Tap	-
KFQ18_RS25170 (91714)	91714..92571	+	858	WP_000130970.1	incFII family plasmid replication initiator RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	paa	1..92777	92777

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T201920 WP_001312851.1 NZ_CP074358:90723-90872 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T201920 NZ_CP099037:829800-830207 [Citrobacter freundii]
GTGGTCCAGTGGCAATCTGATTTACGCGTATCATGGCGCGCGCAGTGGCTTTCACTGCTAATTCATGGGCTGGTCGCGGT
GTTTATTTTATTGATGCCCTGGCCATTGAGCTACACCCCATTATGGCTGGTTTTGCTCTCTCTGGTGGTATTCGATAGCG
TTCGCAGTCAGCGTCGAATCAACGCTTGTCAGGGTGAGATTCGGCTGTTGATGGATGGTCGTCTGCGCTGGCAAGGGCAA
GAGTGGTCTATCGTTAGCGCCCCCTGGATGGTTAAAACAGGCATGATGCTGCGTTTGCGCTCTGATGCCGGAAAACGTCA
ACATTTGTGGCTGGCGGCAGACAGTATGGATGATGCTGAGTGGCGGGATTTACGGCGGTTGATGCTGCAAAAGGCGAAGC
AGAAGTAG

Antitoxin

Nucleotide

• Length: 62 bp

>AT201920 NZ_CP074358:c90679-90618 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin