Detailed information of TA system TA201917

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 54002..54271
• Replicon: plasmid unnamed2
• Accession: NZ_CP074358
• Organism: Escherichia coli strain XJ6.1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KFQ18_RS24940
• Protein ID: WP_001372321.1
• Coordinates: 54146..54271 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	54002..54067 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFQ18_RS24895	49036..49296	+	261	WP_072250151.1	hypothetical protein	-
KFQ18_RS24900	49537..49743	+	207	WP_000275853.1	hypothetical protein	-
KFQ18_RS24905	49769..50308	+	540	WP_000290838.1	single-stranded DNA-binding protein	-
KFQ18_RS24910	50371..50604	+	234	WP_000005990.1	DUF905 family protein	-
KFQ18_RS24915	50670..52628	+	1959	WP_275877718.1	ParB/RepB/Spo0J family partition protein	-
KFQ18_RS24920	52683..53117	+	435	WP_001470779.1	conjugation system SOS inhibitor PsiB	-
KFQ18_RS24925	53114..53876	+	763	Protein_60	plasmid SOS inhibition protein A	-
KFQ18_RS24930	53845..54033	-	189	WP_001299721.1	hypothetical protein	-
-	53845..54069	+	225	NuclAT_0	-	-
-	53845..54069	+	225	NuclAT_0	-	-
-	53845..54069	+	225	NuclAT_0	-	-
-	53845..54069	+	225	NuclAT_0	-	-
-	54002..54067	-	66	-	-	Antitoxin
KFQ18_RS24935	54055..54204	+	150	Protein_62	plasmid maintenance protein Mok	-
KFQ18_RS24940	54146..54271	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
KFQ18_RS24945	54491..54721	+	231	WP_001426396.1	hypothetical protein	-
KFQ18_RS24950	54719..54892	-	174	Protein_65	hypothetical protein	-
KFQ18_RS24955	54963..55169	+	207	WP_275877719.1	single-stranded DNA-binding protein	-
KFQ18_RS24960	55194..55481	+	288	WP_000107548.1	hypothetical protein	-
KFQ18_RS24965	55601..56421	+	821	Protein_68	DUF932 domain-containing protein	-
KFQ18_RS24970	56718..57320	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
KFQ18_RS24975	57641..58024	+	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KFQ18_RS24980	58211..58900	+	690	WP_000283384.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	paa	1..92777	92777

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T201917 WP_001372321.1 NZ_CP074358:54146-54271 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T201917 NZ_CP098926:c4041947-4041645 [Halomonas sp. MS1]
ATGGCTGAAGTAATTTGGACAGAGCCTGCCCTTCAAGAACTGGATGCCATCGCTGAGTACATTGCTCTGGATAATCCTGC
TGCCGCAAGCCATCTGGTCAAAGACGTTTTCGATAAGACCGAGCGTTTGGAAAATTTCCCCCAATCCGGACGGGTTCCTC
CTGAGCTCCCCAATTCGGTATACAGGGAGATAGTGGTTCTACCATGTCGCATTTTTTATCGTGAGGATGAGAAACAGGTT
CTGGTCCTTTATGTTATGCGAGAGGAACGGCAGCTTCGTGCGTACATGCTTGGGAACAGCTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT201917 NZ_CP074358:c54067-54002 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin