Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2477521..2478083 Replicon chromosome
Accession NZ_CP074356
Organism Escherichia coli strain XJ6.1

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag KFQ18_RS11975 Protein ID WP_000605676.1
Coordinates 2477521..2477799 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag KFQ18_RS11980 Protein ID WP_000781370.1
Coordinates 2477799..2478083 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFQ18_RS11950 (2473108) 2473108..2473539 - 432 WP_275877211.1 peroxiredoxin OsmC -
KFQ18_RS11955 (2473884) 2473884..2474099 + 216 WP_000495766.1 biofilm-dependent modulation protein -
KFQ18_RS11960 (2474201) 2474201..2474338 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
KFQ18_RS11965 (2474495) 2474495..2476192 + 1698 WP_275877212.1 malate dehydrogenase -
KFQ18_RS11970 (2476326) 2476326..2477336 + 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
KFQ18_RS11975 (2477521) 2477521..2477799 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KFQ18_RS11980 (2477799) 2477799..2478083 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
KFQ18_RS11985 (2478193) 2478193..2478651 - 459 WP_000526135.1 IS200/IS605-like element IS200C family transposase -
KFQ18_RS11990 (2478846) 2478846..2479499 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
KFQ18_RS11995 (2479492) 2479492..2480376 - 885 WP_001240582.1 formate dehydrogenase N subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2478193..2478651 458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T201905 WP_000605676.1 NZ_CP074356:2477521-2477799 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T201905 NZ_CP098834:c4143158-4142871 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGTTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCCCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT201905 WP_000781370.1 NZ_CP074356:2477799-2478083 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT201905 NZ_CP098834:c4142884-4142582 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References