Detailed information of TA system TA201866

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3171730..3172454
• Replicon: chromosome
• Accession: NZ_CP074352
• Organism: Siccibacter colletis strain YSD YN2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KFZ77_RS15315
• Protein ID: WP_264384674.1
• Coordinates: 3171730..3172041 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KFZ77_RS15320
• Protein ID: WP_264384675.1
• Coordinates: 3172038..3172454 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFZ77_RS15295 (KFZ77_15275)	3166820..3168691	+	1872	WP_264384670.1	amidohydrolase	-
KFZ77_RS15300 (KFZ77_15280)	3168696..3169103	+	408	WP_264384671.1	DoxX family protein	-
KFZ77_RS15305 (KFZ77_15285)	3169100..3170722	+	1623	WP_264384672.1	MFS transporter	-
KFZ77_RS15310 (KFZ77_15290)	3170771..3171592	+	822	WP_264384673.1	alpha/beta hydrolase	-
KFZ77_RS15315 (KFZ77_15295)	3171730..3172041	+	312	WP_264384674.1	type II toxin-antitoxin system HigB family toxin	Toxin
KFZ77_RS15320 (KFZ77_15300)	3172038..3172454	+	417	WP_264384675.1	helix-turn-helix domain-containing protein	Antitoxin
KFZ77_RS15325 (KFZ77_15305)	3172610..3173311	+	702	WP_264384676.1	Fic family protein	-
KFZ77_RS15330 (KFZ77_15310)	3173506..3173808	-	303	WP_264384677.1	putative quinol monooxygenase	-
KFZ77_RS15335 (KFZ77_15315)	3173851..3174486	-	636	WP_264384678.1	glutaredoxin 2	-
KFZ77_RS15340 (KFZ77_15320)	3174695..3175687	+	993	WP_264384679.1	LacI family DNA-binding transcriptional regulator	-
KFZ77_RS15345 (KFZ77_15325)	3175714..3176718	+	1005	WP_264384680.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12374.25 Da
• Isoelectric Point: 10.3760

>T201866 WP_264384674.1 NZ_CP074352:3171730-3172041 [Siccibacter colletis]
MHIVSRAPFEAATRLFPGQAAALDDVYRTLKRECYTSPDEMKKRFPSLDRMKYRAKWWVINVGGGHLRIMFFADFERARI
FIKHISSHAEYDRLTDFYRRNRE

Nucleotide

• Length: 312 bp

>T201866 NZ_CP098808:407825-408334 [Ensifer adhaerens]
ATGGCGGTTGGTGCGCCGGTGCGCCTCTGGCGCGCCTATGTGCCGCGCTGGGCGCATGCGCCGCTCTCGGGCGAAGGGGC
AGGGCGGTACGGTGGGAGGTGGAATCCCGTGGGAACGCCGACGATCTATGCCGCCCGCGAGCTCTCGACCGCCTGGGCCG
AATACAATCAGGGTTTCGTCCAACACCCGGCGCTGATCGCCCAGCTTGAACTCCGCTTTGCCCGTCTTGCCGATCTGACG
GCGCCGGAGATCCTTTCCGACCTTGGTCTCACCGAAGAAATCCACCGATGCGAATGGCGCGATGCGCTCGATCGTGGTCT
GGTGCCGGAGACGCACCGCCTGCAACTGGCGTTGAGCGACCGAGGCTTCGATGGCCTGATCTATCCGTCCTTCATGTCGC
CCGGCGGCACCTGCGTCGCGCTGTGGCACTGGAACGCGGGCGAGGGTTCCGAACTGAAGGTTATCGATCCCGAAGGGCGC
CTGCCGAAAACGCCGGCGTCCTGGCTCTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15436.79 Da
• Isoelectric Point: 4.7878

>AT201866 WP_264384675.1 NZ_CP074352:3172038-3172454 [Siccibacter colletis]
MSYAEAIEAANSLVKIVPILGSSPGRKDYEDALLLIEYLIEYEPDSPLVEMLSARIDRYERTAPEFAAFNARTAAIPAGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILKGDRTLTLKHMQALAKRFDLPVSAFIE

Nucleotide

• Length: 417 bp

>AT201866 NZ_CP098808:407454-407825 [Ensifer adhaerens]
ATGGCTGCTGTCGGTCTCAACATCTCCACCGCCCACTACGGCGAGAGCCATTCGTCTTTCCTGTCCGCTCGGCTGGTTGC
CGATCGCCTTGGCGTGACGCTTGCCGAACTCGCGAAGCTGATCGGTGTCGCGCGCAACACGCTGACGGCGAAGTCCAGTG
CCCGAAAGGTCGATGCGGCGTTGAGCAGCGTCGTCCGTATCCTTGCCATGGCCGGCGAAATGGCGGGCGACGACAGCCGC
GCCGTCATCTGGTTCAAGCACCAGCCGATCCCCGGCTGGGCCGGCAAGACCGCCTATGATCTCGTCGGCGAGGGCAAGGC
CGACAAGGTGCTCGCCTATCTCGAAGCCGTGCGTTCCGGTGTCTACGCCTGA