Detailed information of TA system TA201848

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 579056..579710
• Replicon: plasmid unnamed
• Accession: NZ_CP074351
• Organism: Pantoea dispersa strain YSD_J2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KFZ74_RS22755
• Protein ID: WP_238562147.1
• Coordinates: 579056..579427 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A246NDM6
• Locus tag: KFZ74_RS22760
• Protein ID: WP_021508926.1
• Coordinates: 579411..579710 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFZ74_RS22735 (KFZ74_22715)	574333..575712	+	1380	WP_238562146.1	heavy metal sensor histidine kinase	-
KFZ74_RS22740 (KFZ74_22720)	575742..576395	-	654	WP_058770319.1	oxygen-insensitive NAD(P)H nitroreductase	-
KFZ74_RS22745 (KFZ74_22725)	576500..577501	-	1002	WP_021508931.1	zinc-binding alcohol dehydrogenase family protein	-
KFZ74_RS22750 (KFZ74_22730)	577638..578555	+	918	WP_058759023.1	LysR family transcriptional regulator	-
KFZ74_RS22755 (KFZ74_22735)	579056..579427	+	372	WP_238562147.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KFZ74_RS22760 (KFZ74_22740)	579411..579710	+	300	WP_021508926.1	XRE family transcriptional regulator	Antitoxin
KFZ74_RS22765 (KFZ74_22745)	579788..580255	-	468	WP_058759022.1	hypothetical protein	-
KFZ74_RS22770 (KFZ74_22750)	580441..581370	-	930	WP_059009262.1	ABC transporter substrate-binding protein	-
KFZ74_RS22775 (KFZ74_22755)	581380..582114	-	735	WP_058776076.1	ABC transporter permease	-
KFZ74_RS22780 (KFZ74_22760)	582098..582814	-	717	WP_238562148.1	ABC transporter ATP-binding protein	-
KFZ74_RS22785 (KFZ74_22765)	582811..583779	-	969	WP_238562149.1	HesA/MoeB/ThiF family protein	-
KFZ74_RS22790 (KFZ74_22770)	583790..584548	-	759	WP_238562150.1	thiazole synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iroN	1..710238	710238

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (10-88)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 14274.45 Da
• Isoelectric Point: 10.0560

>T201848 WP_238562147.1 NZ_CP074351:579056-579427 [Pantoea dispersa]
VWQVEATDRFCKWLQAQDEALRLDVLAALKLLAQDGPHLGRPFVDTLMLSRVPNMKELRVQSKGRPIRSFFVFDPRRHAI
VLCAGNKQGKNQKRFYQQMLRIAETEYHHHLNAIGETHNENLG

Nucleotide

• Length: 372 bp

>T201848 NZ_CP098792:c92021-91734 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11265.00 Da
• Isoelectric Point: 6.7255

>AT201848 WP_021508926.1 NZ_CP074351:579411-579710 [Pantoea dispersa]
MKTLDEMLAALTPEQQHQVDERVSQMRLEYQLYQLREQMQRTQRQQAQAMHIAQSSVAAMEARGAELKISTLKRYIEALG
GELTLGVKMPGGQHVDLII

Nucleotide

• Length: 300 bp

>AT201848 NZ_CP098792:c91747-91433 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGATTGGAGCAGTGCTGTACGCC
AAAATCAATTTTACCGCCCAGTGAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA

Structures

Antitoxin

• AlphaFold DB: A0A246NDM6