Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 177797..178477 Replicon chromosome
Accession NZ_CP074350
Organism Pantoea dispersa strain YSD_J2

Toxin (Protein)


Gene name higB Uniprot ID A0A246NHY2
Locus tag KFZ74_RS00825 Protein ID WP_059013049.1
Coordinates 177797..178096 (+) Length 100 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A246NHX0
Locus tag KFZ74_RS00830 Protein ID WP_058775157.1
Coordinates 178145..178477 (+) Length 111 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFZ74_RS00810 (KFZ74_00815) 174274..174768 + 495 WP_021507883.1 GNAT family N-acetyltransferase -
KFZ74_RS00815 (KFZ74_00820) 174993..175907 - 915 WP_021507882.1 sugar ABC transporter substrate-binding protein -
KFZ74_RS00820 (KFZ74_00825) 176343..177737 + 1395 WP_159378007.1 MFS transporter -
KFZ74_RS00825 (KFZ74_00830) 177797..178096 + 300 WP_059013049.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KFZ74_RS00830 (KFZ74_00835) 178145..178477 + 333 WP_058775157.1 HigA family addiction module antitoxin Antitoxin
KFZ74_RS00835 (KFZ74_00840) 178569..179732 + 1164 WP_021507880.1 HD-GYP domain-containing protein -
KFZ74_RS00840 (KFZ74_00845) 179841..179930 + 90 WP_021507879.1 K(+)-transporting ATPase subunit F -
KFZ74_RS00845 (KFZ74_00850) 179930..181609 + 1680 WP_150361673.1 potassium-transporting ATPase subunit KdpA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(6-95)

Antitoxin

Cro/C1-type helix-turn-helix domain

(18-69)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11503.22 Da        Isoelectric Point: 10.4912

>T201840 WP_059013049.1 NZ_CP074350:177797-178096 [Pantoea dispersa]
MGSIRAFRQAWLAQFYTHGTPHAQIPRAIESALARKLDIIHAATSHQDLRSPPGNRFEALKPPLLGYYSIRVNAQYRLIF
QWAKGEAWDLYLDPHRYKK

Download         Length: 300 bp

>T201840 NZ_CP098778:c3686767-3686357 [Enterobacter hormaechei]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGTCTGGTGGCGGC
ACTGGTGTTATTGGTGCCGTGGCCGCTTAGCTATACCCCTCTGTGGTTGCTATTGCTGTCGTTTGTGGTGTTTGACAGCG
TGCGCAGCCAGCGTCGAATCAACGCCCGTCAGGGTGAAATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAGATGATGGGGACGCCGTGGATGCTTAATACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGATAACCGTCG
CCAGCACCTGTGGCTGGCCGCAGACAGTATGGATCCTGCGGAGTGGCGAGATCTGCGCCGGTTGATCGTGCAACAGCCGA
CGCAGGAGTAA

Antitoxin


Download         Length: 111 a.a.        Molecular weight: 12751.58 Da        Isoelectric Point: 7.2039

>AT201840 WP_058775157.1 NZ_CP074350:178145-178477 [Pantoea dispersa]
MKQAMRKPTSVGDVLQYEFLEPLNLKVNDLAEMLNVHRNTVSALVNNNRKLSTEMAYRLARVFATSIEFWLNLQRHLDQW
EVENDARTQEELSRVMTLSAFLAQRGTQVA

Download         Length: 333 bp

>AT201840 NZ_CP098778:c3687014-3686748 [Enterobacter hormaechei]
ATGGATATTAACAACAAAGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAATATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A246NHY2

Antitoxin

Source ID Structure
AlphaFold DB A0A246NHX0

References