Detailed information of TA system TA201780

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 115037..115538
• Replicon: plasmid pJBIWA002_1
• Accession: NZ_CP074179
• Organism: Enterobacter asburiae strain JBIWA002

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: KGP20_RS24660
• Protein ID: WP_123061045.1
• Coordinates: 115037..115312 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: KGP20_RS24665
• Protein ID: WP_103061436.1
• Coordinates: 115293..115538 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP20_RS24630 (KGP20_24505)	111137..111603	+	467	Protein_112	transposase	-
KGP20_RS24635 (KGP20_24510)	111682..111942	+	261	WP_223582592.1	hypothetical protein	-
KGP20_RS24640 (KGP20_24515)	112283..113452	+	1170	Protein_114	IS256 family transposase	-
KGP20_RS24645 (KGP20_24520)	113524..114330	+	807	Protein_115	IS3 family transposase	-
KGP20_RS24650 (KGP20_24525)	114404..114721	-	318	WP_123061047.1	hypothetical protein	-
KGP20_RS24655 (KGP20_24530)	114718..114924	-	207	WP_123061046.1	hypothetical protein	-
KGP20_RS24660 (KGP20_24535)	115037..115312	-	276	WP_123061045.1	Txe/YoeB family addiction module toxin	Toxin
KGP20_RS24665 (KGP20_24540)	115293..115538	-	246	WP_103061436.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KGP20_RS24670 (KGP20_24545)	115780..116025	+	246	WP_123061044.1	hypothetical protein	-
KGP20_RS24675 (KGP20_24550)	116865..117239	-	375	WP_143345794.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KGP20_RS24680 (KGP20_24555)	117707..118162	+	456	WP_143345793.1	transglycosylase SLT domain-containing protein	-
KGP20_RS24685 (KGP20_24560)	118174..118692	-	519	WP_223540708.1	antirestriction protein	-
KGP20_RS24690 (KGP20_24565)	119159..119989	-	831	WP_143345791.1	N-6 DNA methylase	-
KGP20_RS24695 (KGP20_24570)	120032..120244	-	213	WP_245403088.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaFRI-5	clpV/tssH / hcp/tssD / vipB/tssC / vipA/tssB / vipA/tssB / vipB/tssC / hcp/tssD / clpV/tssH	1..243962	243962

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10891.43 Da
• Isoelectric Point: 10.4471

>T201780 WP_123061045.1 NZ_CP074179:c115312-115037 [Enterobacter asburiae]
MSKWNIKFTRNGADDLSYWRDTDKKKFERIRQLLRSIEADPLTGIGKPERLRHHKDPALYSRRIDQSHRLVYSIKSGEII
IYAARYHYGDK

Nucleotide

• Length: 276 bp

>T201780 NZ_CP074179:c115312-115037 [Enterobacter asburiae]
TTGTCGAAGTGGAATATTAAGTTCACCAGGAACGGAGCTGATGACCTTAGCTACTGGCGTGATACTGACAAAAAGAAGTT
TGAGCGTATCCGGCAGCTGTTGCGCAGCATCGAGGCTGATCCGCTGACAGGTATCGGAAAACCCGAACGGCTGCGGCACC
ACAAAGATCCGGCGCTCTATTCCCGCCGGATTGATCAGAGTCACCGTCTGGTCTATTCAATCAAGAGCGGAGAAATCATC
ATCTACGCAGCCCGTTATCACTATGGTGACAAATAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9289.43 Da
• Isoelectric Point: 4.5069

>AT201780 WP_103061436.1 NZ_CP074179:c115538-115293 [Enterobacter asburiae]
MHAINFTDARKHFADTMKRVTDDAEPVRVMRRDAPDIVMIDAGEYEALIETVYLFSNPANAAHINESMKQAERGEFVEVE
Y

Nucleotide

• Length: 246 bp

>AT201780 NZ_CP074179:c115538-115293 [Enterobacter asburiae]
ATGCATGCAATCAATTTTACTGATGCCCGTAAACATTTTGCTGACACCATGAAGCGCGTCACTGATGATGCTGAACCTGT
ACGCGTCATGCGTCGTGACGCACCTGATATTGTTATGATCGATGCAGGAGAGTATGAGGCGCTAATCGAAACGGTCTATC
TGTTCAGCAATCCGGCGAACGCCGCGCATATCAATGAGTCAATGAAACAGGCGGAGCGCGGCGAATTTGTCGAAGTGGAA
TATTAA