Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3128558..3129218 Replicon chromosome
Accession NZ_CP074170
Organism Enterobacter sp. JBIWA003

Toxin (Protein)


Gene name higB Uniprot ID A0A330EN37
Locus tag KGP25_RS15085 Protein ID WP_069718932.1
Coordinates 3128844..3129218 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A330EP84
Locus tag KGP25_RS15080 Protein ID WP_069718933.1
Coordinates 3128558..3128860 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KGP25_RS15060 (KGP25_15010) 3124401..3126590 + 2190 WP_111968096.1 TonB-dependent siderophore receptor -
KGP25_RS15070 (KGP25_15020) 3127021..3127410 - 390 WP_045908132.1 RidA family protein -
KGP25_RS15075 (KGP25_15025) 3127552..3128484 + 933 WP_223539216.1 LysR family transcriptional regulator -
KGP25_RS15080 (KGP25_15030) 3128558..3128860 - 303 WP_069718933.1 XRE family transcriptional regulator Antitoxin
KGP25_RS15085 (KGP25_15035) 3128844..3129218 - 375 WP_069718932.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KGP25_RS15090 (KGP25_15040) 3129398..3129535 - 138 Protein_2951 transcriptional regulator -
KGP25_RS15095 3129528..3129731 + 204 Protein_2952 glutathione S-transferase -
KGP25_RS15100 (KGP25_15045) 3129762..3130712 - 951 WP_028013741.1 HTH-type transcriptional regulator Cbl -
KGP25_RS15105 (KGP25_15050) 3130809..3131726 - 918 WP_223539217.1 nitrogen assimilation transcriptional regulator NAC -
KGP25_RS15115 (KGP25_15060) 3132257..3133153 - 897 WP_196365069.1 L,D-transpeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-106)

Antitoxin

Cro/C1-type helix-turn-helix domain

(33-79)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14318.33 Da        Isoelectric Point: 9.2642

>T201747 WP_069718932.1 NZ_CP074170:c3129218-3128844 [Enterobacter sp. JBIWA003]
VWTINTTDRFDCWFRSLDDTDRACVLAALLVLREKGPALSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFDPNRTGI
VLCAGNKAGNERRFYDEMLLVADREFTHWLNTLKERTLRDGQKP

Download         Length: 375 bp

>T201747 NZ_CP098741:c3411255-3410980 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGAATGCCTCCGGCGTCAGCGTCCGCCATATCAACAGCGAAACCTGCATGACCGCCTGTTACTCACAAATCCCCAGCCA
GCACCTTAAGGGTGACTGGCAGGAAGAAGCGGGATTTGAGACCGGGCACGGCGTCACCGTGAAGATCTCCGAGGGGTGTT
TGATCCTGATTGCAGAGACGGACGAAGTGAGGGATCTACGGAAGGAGCTTTATCAGGTGAAAAAGTCGATGAAGCATATT
AAGGCGGGGGTAAATAATGTGGTGAACGGGGATTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 10995.81 Da        Isoelectric Point: 5.7251

>AT201747 WP_069718933.1 NZ_CP074170:c3128860-3128558 [Enterobacter sp. JBIWA003]
MGRNLEQLIADEKPDVVASAQAMAADILLNIHLAELREKVQKTQVEMAQALGIKQPTVAIMEKPGRDLKLSSLKRYVEAA
GGKLKLDVELPDGSHYEFVL

Download         Length: 303 bp

>AT201747 NZ_CP098741:3410990-3411412 [Salmonella enterica subsp. enterica serovar Typhimurium]
TTCACCACATTATTTACCCCCGCCTTAATATGCTTCATCGACTTTTTCACCTGATAAAGCTCCTTCCGTAGATCCCTCAC
TTCGTCCGTCTCTGCAATCAGGATCAAACACCCCTCGGAGATCTTCACGGTGACGCCGTGCCCGGTCTCAAATCCCGCTT
CTTCCTGCCAGTCACCCTTAAGGTGCTGGCTGGGGATTTGTGAGTAACAGGCGGTCATGCAGGTTTCGCTGTTGATATGG
CGGACGCTGACGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTATTTATCCGCCGGATGCTGGCTGATTGTGGA
GCTCAGGGTGAGTGAGTATGGGCGCGACATCCTGGCACCGCGCCTCCCTCTCCCCGGCCAGCCCCGGCCGGTGATGAGCA
GCCGGCTGCCGGGGCCGTATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330EN37

Antitoxin

Source ID Structure
AlphaFold DB A0A330EP84

References