Detailed information of TA system TA201716

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 479921..480549
• Replicon: chromosome
• Accession: NZ_CP074168
• Organism: Serratia ureilytica strain JBIWA004

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8G2G488
• Locus tag: KGP23_RS02210
• Protein ID: WP_016930174.1
• Coordinates: 480259..480549 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A8B4GQY7
• Locus tag: KGP23_RS02205
• Protein ID: WP_015379150.1
• Coordinates: 479921..480244 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP23_RS02180 (KGP23_02175)	474932..476167	-	1236	WP_223524454.1	serine/threonine transporter SstT	-
KGP23_RS02185 (KGP23_02180)	476431..477399	-	969	WP_025304345.1	TerC family protein	-
KGP23_RS02190 (KGP23_02185)	477652..478383	-	732	WP_033644531.1	glutamine amidotransferase	-
KGP23_RS02195 (KGP23_02190)	478385..479365	-	981	WP_072626939.1	Gfo/Idh/MocA family oxidoreductase	-
KGP23_RS02200 (KGP23_02195)	479416..479928	-	513	WP_042785417.1	M48 family metallopeptidase	-
KGP23_RS02205 (KGP23_02200)	479921..480244	-	324	WP_015379150.1	HigA family addiction module antitoxin	Antitoxin
KGP23_RS02210 (KGP23_02205)	480259..480549	-	291	WP_016930174.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KGP23_RS02215 (KGP23_02210)	480780..481919	+	1140	WP_033651272.1	23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG	-
KGP23_RS02220 (KGP23_02215)	482113..484134	-	2022	WP_223524456.1	NADPH-dependent 2,4-dienoyl-CoA reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (24-71)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11645.30 Da
• Isoelectric Point: 10.2782

>T201716 WP_016930174.1 NZ_CP074168:c480549-480259 [Serratia ureilytica]
MIKSFRDRYLEQFYLEGRRSRLIPGALERQLARKLDMLAAAQQERDLHHPTGNYYKRLSGPFQGWSSLRVNMHWRLMFQW
RHDAAEKIYLDPHQDT

Nucleotide

• Length: 291 bp

>T201716 NZ_CP098724:c3006847-3006425 [Yersinia ruckeri]
GTGGCCCAGTGGCGATGTGATCTGCGGGTTTCATGGCGCACCCAACTTTTCTCACTATTGGTACACGGTGTTCTGGTATT
ACTGACACTGGTGGTACCTTGGCCTGACGGATTTACTCCGCTATGGCTTATATTGCTGACGCTGGTGGTGTTTGAATGCA
TTCGCAGCCAGAAGAACATTACCTCTCGACAGGGAGAGATCCGGCTTAAAGCGGGAAATCTGTTGATTTGGAAGTGGCAA
GAGTGGACGTTGGTGCGGCCGCCGTGGATTACCCGCTACGGCGTATTATTATCGTTACAAGGCGCTGGCAGCCGCCGCCG
AAAACGTATTTGGCTAGCCTCCGACAGCATGTCGGAAGAAAAATGGCGCGAGTTGTGTCTGATATTACGACATACTTTTG
ATTCTGCGGCAGGCCGTGAGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12141.78 Da
• Isoelectric Point: 6.2285

>AT201716 WP_015379150.1 NZ_CP074168:c480244-479921 [Serratia ureilytica]
MLETTTREPTTVGEVLQEEYLTPLGIGQEELAKVLGVTRATVNRLCNGHRRMSVAEANLFADLFETTADFWLNLQAAHDR
WEARQADAQRVRLRPIKEILGRNITHA

Nucleotide

• Length: 324 bp

>AT201716 NZ_CP098724:c3007094-3006828 [Yersinia ruckeri]
ATGGAAATTGATAACAAAGCCCGAATCCATTGGGCATGCCGCCGGGGTATGCGTGAACTGGATATCTCCATAATGCCATT
TTTCGAACATGAATATGACACGCTAAACGATAATGATAAGCGCGTATTTATCCGCTTGCTGACGAGTGATGATCCTGATT
TATTCAATTGGTTGATGAACCACGGCGAGCCGCAGGATAGCGAACTGAAACGAATGGTTAAACTGATTCAGACACGAAAT
AAAGCCCGTGGCCCAGTGGCGATGTGA

Structures

Antitoxin

• AlphaFold DB: A0A8B4GQY7