Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 416559..417289 Replicon chromosome
Accession NZ_CP074168
Organism Serratia ureilytica strain JBIWA004

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag KGP23_RS01890 Protein ID WP_108682058.1
Coordinates 416978..417289 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KGP23_RS01885 Protein ID WP_033644489.1
Coordinates 416559..416981 (-) Length 141 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KGP23_RS01870 (KGP23_01865) 412544..413284 + 741 WP_033651249.1 KDGP aldolase family protein -
KGP23_RS01875 (KGP23_01870) 413372..414586 + 1215 WP_223524423.1 lactonase family protein -
KGP23_RS01880 (KGP23_01875) 414638..416554 + 1917 WP_223524424.1 PRD domain-containing protein -
KGP23_RS01885 (KGP23_01880) 416559..416981 - 423 WP_033644489.1 helix-turn-helix domain-containing protein Antitoxin
KGP23_RS01890 (KGP23_01885) 416978..417289 - 312 WP_108682058.1 type II toxin-antitoxin system HigB family toxin Toxin
KGP23_RS01895 (KGP23_01890) 417584..417856 - 273 WP_004937034.1 PTS phosphocarrier protein NPr -
KGP23_RS01900 (KGP23_01895) 417853..418707 - 855 WP_015379177.1 RNase adapter RapZ -
KGP23_RS01905 (KGP23_01900) 418835..419296 - 462 WP_016930212.1 PTS IIA-like nitrogen regulatory protein PtsN -
KGP23_RS01910 (KGP23_01905) 419452..419739 - 288 WP_004937038.1 ribosome hibernation promoting factor -
KGP23_RS01915 (KGP23_01910) 419763..421196 - 1434 WP_004937039.1 RNA polymerase factor sigma-54 -
KGP23_RS01920 (KGP23_01915) 421249..421974 - 726 WP_042785437.1 LPS export ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(81-135)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12255.12 Da        Isoelectric Point: 10.0957

>T201715 WP_108682058.1 NZ_CP074168:c417289-416978 [Serratia ureilytica]
MHIISREPFNNATKQYPNEATALEAIYKALRRGEFRTPDELKALFPSLDRMKYKEKWWVIDVGGNHLRILFFASLETQKV
FIKHIVSHAEYDKLMQHYRRSSS

Download         Length: 312 bp

>T201715 NZ_CP098724:2812095-2812424 [Yersinia ruckeri]
ATGCAAACCTTATCATCACACCCGACACGGGCGTCTCAGCCCTGCCTGTCACCCGTTGAAATCTGGCAGCTGCTGCTGAC
TCATTTGTTGTCGCAGCACTATGGCCTGACGCTGAATGACACACCGTTCATTAATGAAACCACCATCCGGGAACATATTG
ATGCTGGAGTATCGCTCAGCGATGCGGTGAACTTTCTGGTAGAAAAATATGGGCTTGTCCGTATCGACCGGAAGGGATTT
TCGTGGCAAGAACAGACCCCATATATTTCCGTAGTTGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACCAA
CGTGAAATAA

Antitoxin


Download         Length: 141 a.a.        Molecular weight: 15784.13 Da        Isoelectric Point: 5.2139

>AT201715 WP_033644489.1 NZ_CP074168:c416981-416559 [Serratia ureilytica]
MITDAIKAADALLRAVPLLQGSHAQQDYREALELVEYLLENDERHPLIDMLAKKIAEYEDNSAEFTAFNQRIAQLPQGVA
ALRVLMEQHQLSQSDFEHEIGKKSLVNQILNGKRSLTIPHIMALAKRFNLPPALFLPEAD

Download         Length: 423 bp

>AT201715 NZ_CP098724:2811691-2812056 [Yersinia ruckeri]
ATGAACAATCACTCAGAATCCGTTACCAAGCCGGAGAATCCCGCCTGCCAGCAGTGGGGACTAAAGAGTACGATCACACC
CTGTTTTGGAGCTCGTCTGGTGCTGGAAGGCAACCGCTTGCATTTTCTCGCTGACCGGGCCGGATTCAACGGTGCTTTCA
GCGACGATGACGCGCTGCACCTAGATCATGCCTTTCCGTTGATACTCAAGCAACTGGAGCTGATGCTCACCAGCGGAGAA
CTCTCTCCCCGGCATCAGCACTGCGTCACGCTATATCACAACGGACTCACTTGCGAAGCCGACACACTCGGCTCCTGTGG
CTATGTCTACATTGCTATTTATCCTGACCAGACTGAGCCGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References