Detailed information of TA system TA201710

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 165152..165653
• Replicon: plasmid pJBIWA005_1
• Accession: NZ_CP074160
• Organism: Enterobacter sp. JBIWA005

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: KGP22_RS23230
• Protein ID: WP_123061045.1
• Coordinates: 165378..165653 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: KGP22_RS23225
• Protein ID: WP_103061436.1
• Coordinates: 165152..165397 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP22_RS23195 (KGP22_23075)	160446..160658	+	213	WP_245403088.1	hypothetical protein	-
KGP22_RS23200 (KGP22_23080)	160701..161531	+	831	WP_143345791.1	N-6 DNA methylase	-
KGP22_RS23205 (KGP22_23085)	161998..162516	+	519	WP_223540708.1	antirestriction protein	-
KGP22_RS23210 (KGP22_23090)	162528..162983	-	456	WP_143345793.1	transglycosylase SLT domain-containing protein	-
KGP22_RS23215 (KGP22_23095)	163451..163825	+	375	WP_143345794.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KGP22_RS23220 (KGP22_23100)	164665..164910	-	246	WP_123061044.1	hypothetical protein	-
KGP22_RS23225 (KGP22_23105)	165152..165397	+	246	WP_103061436.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KGP22_RS23230 (KGP22_23110)	165378..165653	+	276	WP_123061045.1	Txe/YoeB family addiction module toxin	Toxin
KGP22_RS23235 (KGP22_23115)	165766..165972	+	207	WP_123061046.1	hypothetical protein	-
KGP22_RS23240 (KGP22_23120)	165969..166286	+	318	WP_123061047.1	hypothetical protein	-
KGP22_RS23245 (KGP22_23125)	166360..167166	-	807	Protein_182	IS3 family transposase	-
KGP22_RS23250 (KGP22_23130)	167238..168407	-	1170	Protein_183	IS256 family transposase	-
KGP22_RS23255 (KGP22_23135)	168748..169008	-	261	WP_223582592.1	hypothetical protein	-
KGP22_RS23260 (KGP22_23140)	169087..169553	-	467	Protein_185	transposase	-
KGP22_RS23265 (KGP22_23145)	170143..170643	+	501	WP_123061084.1	Ail/Lom family outer membrane beta-barrel protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaFRI-5	vipA/tssB / vipB/tssC / hcp/tssD / clpV/tssH	1..191037	191037

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10891.43 Da
• Isoelectric Point: 10.4471

>T201710 WP_123061045.1 NZ_CP074160:165378-165653 [Enterobacter sp. JBIWA005]
MSKWNIKFTRNGADDLSYWRDTDKKKFERIRQLLRSIEADPLTGIGKPERLRHHKDPALYSRRIDQSHRLVYSIKSGEII
IYAARYHYGDK

Nucleotide

• Length: 276 bp

>T201710 NZ_CP098724:455999-456289 [Yersinia ruckeri]
ATGACCTTTAATATCGACTTTGACGAACGGGCATTAAAAGAATGGCACAAACTAGATAAAACTATCCGAGAGCAATTTAA
AAAGAAACTAAGAAAATTACAGGAAAATCCCTATATTGAGTCAGCACGTTTGCACGGTGATCTGGCTGGTTGTTTCAAAA
TAAAATTACGCGCATCTGGCTTTCGGTTGATTTACCAAGTTATCGATGAGGAGATTGTGATTTTGATCGTTGCTGTTGGT
AAACGGGAAGATGAAAAAGCTTATGATCTTGCCAAGAAGCGAATCCAATAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9289.43 Da
• Isoelectric Point: 4.5069

>AT201710 WP_103061436.1 NZ_CP074160:165152-165397 [Enterobacter sp. JBIWA005]
MHAINFTDARKHFADTMKRVTDDAEPVRVMRRDAPDIVMIDAGEYEALIETVYLFSNPANAAHINESMKQAERGEFVEVE
Y

Nucleotide

• Length: 246 bp

>AT201710 NZ_CP098724:455755-456009 [Yersinia ruckeri]
ATGAGTACTATCCAAATTCGTGTGGATGATGAACTGAAGAAAAGTGCATATCAGGCGTTTGAAAAACTGAATCTTTCGCC
TTCTGATGCACTACGCCTGTTCCTACGTTATGTTGCAGAGAACGAAAAGCTGCCTTTTTCAGAAGTATCCGTCATGGTCA
GTAATCAAGATGAGGATGCTGATATTTTGGCTGTTGTCCGTGAACGTTTGAAAAATCCTGCAAAGCGTATCAGAGTAAAT
ATTGATGACCTTTAA