Detailed information of TA system TA201705

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3639793..3640450
• Replicon: chromosome
• Accession: NZ_CP074159
• Organism: Enterobacter sp. JBIWA005

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KGP22_RS17225
• Protein ID: WP_111965881.1
• Coordinates: 3639793..3640092 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1Z3MZ32
• Locus tag: KGP22_RS17230
• Protein ID: WP_063408808.1
• Coordinates: 3640103..3640450 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP22_RS17215 (KGP22_17120)	3636164..3638362	+	2199	WP_223580325.1	type I secretion system permease/ATPase	-
KGP22_RS17220 (KGP22_17125)	3638373..3639542	+	1170	WP_223582464.1	HlyD family efflux transporter periplasmic adaptor subunit	-
KGP22_RS17225 (KGP22_17130)	3639793..3640092	+	300	WP_111965881.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KGP22_RS17230 (KGP22_17135)	3640103..3640450	+	348	WP_063408808.1	HigA family addiction module antitoxin	Antitoxin
KGP22_RS17235 (KGP22_17140)	3640471..3640989	-	519	WP_223580327.1	hypothetical protein	-
KGP22_RS17240 (KGP22_17145)	3641075..3642256	-	1182	WP_223580329.1	PLP-dependent aminotransferase family protein	-
KGP22_RS17245 (KGP22_17150)	3642277..3643164	-	888	WP_115876110.1	LysR family transcriptional regulator	-
KGP22_RS17250 (KGP22_17155)	3643262..3643864	+	603	WP_223580331.1	short chain dehydrogenase	-
KGP22_RS17255 (KGP22_17160)	3643981..3645360	-	1380	WP_223580333.1	amidohydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3578962..3648118	69156

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-95)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (8-86)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11654.27 Da
• Isoelectric Point: 9.6244

>T201705 WP_111965881.1 NZ_CP074159:3639793-3640092 [Enterobacter sp. JBIWA005]
MISYFRDQWLEDFFLYGRSSNVIPSNLETALARKLDIIRAATSHRDLRSPPGNMYEALNPPLKGYSSIRVNRQYRLVFCW
TEGKAEDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T201705 NZ_CP098723:c1757762-1757304 [Yersinia ruckeri]
GTGATGCTCTACCGGATAGTTCACAACCGTTACCTGGCAACGGCCTGGACCGGATATGGCGCAGAAACCTATGGTGGCCG
CTGGAATCATAAAGGCCATGCGGCGATTTATCTGGCCAGTAGCGTTTCTCTGGCGATGCTCGAAACACTGGTGCATGTAG
AAGATAGCGCGACGCTGAACGATTTTAATCTATTTCAGATAACCATTCCCGATAATGCAATTATGGTACTGCAACAGCAA
GATTGGCCACGTGGGTGGCGAGATGACCCCCCCCCCGTGGCTACCATGGACATCGGCACCGAATGGATTGGTTCAGCATC
ATCTGTCGGTCTGCTGGTACCATCCACGTTAGTCCCTTTGGAAAGAAATATGCTGGTGAATCCTAAACACCGCGATTTCG
CCTCGTGCCTGAAAAGCATCAATCCACTGACGTTCAGTTTTGATCCTCGTCTAAAGTAA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13039.98 Da
• Isoelectric Point: 7.0134

>AT201705 WP_063408808.1 NZ_CP074159:3640103-3640450 [Enterobacter sp. JBIWA005]
MTLQQALRKPTTPGDVLQYEYLEPLNLKISDLAEMLNVHRNTISALVNNNRKLTADMAIKLAKAFDTTIEFWLNLQLNVD
IWEAQSNARTQEELSRIKTVADVMAKRKSGQPDVA

Nucleotide

• Length: 348 bp

>AT201705 NZ_CP098723:c1758222-1757776 [Yersinia ruckeri]
ATGAGAACTTATCACCCAAGCCCGATGGCCAAAGTCAGCGCACTATGGCGTGAGATTGGTCTACCCGCCAGTCGTGGCAC
CGCTTTGGTGGACAGCATAAAACTGGGATTTACTGTGGATGTGATCGACACTATACATTTATGGGCTGATATGCCTAAAT
CTGAAATATTACGCGCTACGGGCATTCCGAGCCGCAGTCTGACGCGCCGTCGTACCCATGATGGGCGTTTTACCCCGGAA
GAAAGCGAACGTATTGCACGGTTTGTCCGGGTTATGGATGCGGCGGTAGATTTGTTCGGTGGTGACCGCACCAAAGCAAC
CGCATGGATGTCGATGCCGGTCAAAGGGCTGGGTAATCGAACGCCCGATTCACTGCTGGAAACCGAAACCGGTGCGTTGG
AAATCTGCGACCTGATTGGTCGTCTCGAACATGGGGTCTTTTCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1Z3MZ32