Detailed information of TA system TA201680

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1661136..1661796
• Replicon: chromosome
• Accession: NZ_CP074154
• Organism: Enterobacter asburiae strain JBIWA007

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KGP18_RS08000
• Protein ID: WP_223536848.1
• Coordinates: 1661136..1661510 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1Z3MX79
• Locus tag: KGP18_RS08005
• Protein ID: WP_029741460.1
• Coordinates: 1661494..1661796 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP18_RS07980 (KGP18_07985)	1657278..1658195	+	918	WP_223536827.1	nitrogen assimilation transcriptional regulator NAC	-
KGP18_RS07985 (KGP18_07990)	1658292..1659242	+	951	WP_223536839.1	HTH-type transcriptional regulator Cbl	-
KGP18_RS07990 (KGP18_07995)	1659286..1659894	-	609	WP_223536842.1	glutathione S-transferase family protein	-
KGP18_RS07995 (KGP18_08000)	1660065..1660958	+	894	WP_223536845.1	LysR substrate-binding domain-containing protein	-
KGP18_RS08000 (KGP18_08005)	1661136..1661510	+	375	WP_223536848.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KGP18_RS08005 (KGP18_08010)	1661494..1661796	+	303	WP_029741460.1	XRE family transcriptional regulator	Antitoxin
KGP18_RS08010 (KGP18_08015)	1661871..1662791	-	921	WP_223536851.1	LysR family transcriptional regulator	-
KGP18_RS08015 (KGP18_08020)	1662933..1663322	+	390	WP_223536853.1	RidA family protein	-
KGP18_RS08020 (KGP18_08025)	1663614..1663871	+	258	WP_223536856.1	antitoxin	-
KGP18_RS08025 (KGP18_08030)	1663872..1664204	+	333	WP_064672767.1	type II toxin-antitoxin system PemK/MazF family toxin	-
KGP18_RS08035 (KGP18_08040)	1664499..1666688	-	2190	WP_223536859.1	TonB-dependent siderophore receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1652594..1664204	11610

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14312.24 Da
• Isoelectric Point: 9.6819

>T201680 WP_223536848.1 NZ_CP074154:1661136-1661510 [Enterobacter asburiae]
VWAINTTDRFDRWFTSLDDTERASVLAALLVLREKGPGLSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFDPNRTGI
VLCAGNKVGNERRFYDEMLLVADREFTHWLNTLKERELRDGKNP

Nucleotide

• Length: 375 bp

>T201680 NZ_CP074154:1661136-1661510 [Enterobacter asburiae]
GTGTGGGCAATTAACACGACGGACAGGTTTGATCGCTGGTTCACTTCGCTTGACGATACCGAACGCGCCAGCGTACTTGC
AGCACTGCTTGTTTTGCGAGAGAAGGGGCCGGGTTTATCGAGACCCTACGCTGATACGCTTAAAGGCTCTCGTCACAGCA
ACATGAAAGAGCTACGTATTCAGAGTAAGGGCGATCCCTTGCGGGCGTTTTTCGCCTTTGATCCAAACCGCACGGGTATC
GTGCTTTGTGCGGGCAATAAGGTCGGCAATGAAAGGCGATTCTACGATGAGATGCTTCTCGTTGCTGACCGGGAATTCAC
TCACTGGCTGAATACATTAAAAGAGAGGGAATTGCGCGATGGGAAGAACCCTTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11050.83 Da
• Isoelectric Point: 5.7316

>AT201680 WP_029741460.1 NZ_CP074154:1661494-1661796 [Enterobacter asburiae]
MGRTLEQLIADEKPEVVASAQAIATDILLNIHLAELREKVQKTQVEMAQALGIRQPTVAVMEKPGRDLKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT201680 NZ_CP074154:1661494-1661796 [Enterobacter asburiae]
ATGGGAAGAACCCTTGAGCAGCTTATAGCGGATGAAAAGCCGGAGGTTGTCGCCAGCGCTCAGGCGATAGCAACGGATAT
TTTGCTCAACATTCATCTTGCTGAGTTACGCGAGAAAGTACAAAAAACGCAGGTAGAAATGGCGCAGGCGTTGGGTATCA
GGCAACCTACCGTTGCGGTAATGGAAAAGCCCGGGCGCGATTTGAAACTCTCTTCTCTCAAGCGCTACGTTGAGGCGGCG
GGCGGAAAGCTGCGGCTTGATGTGGAGCTGCCGGATGGCTCGCACTATGAGTTTGTGCTGTAG

Structures

Antitoxin

• AlphaFold DB: A0A1Z3MX79