Detailed information of TA system TA201677

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 20208..20935
• Replicon: plasmid pJBIWA008_2
• Accession: NZ_CP074151
• Organism: Enterobacter sp. JBIWA008

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KGP24_RS23960
• Protein ID: WP_079899846.1
• Coordinates: 20208..20519 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KGP24_RS23965
• Protein ID: WP_079899847.1
• Coordinates: 20516..20935 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP24_RS23935 (KGP24_23795)	15929..16834	-	906	WP_223563661.1	recombination-associated protein RdgC	-
KGP24_RS23940 (KGP24_23800)	16894..17526	-	633	WP_223563662.1	baseplate protein	-
KGP24_RS23945 (KGP24_23805)	17523..18503	-	981	WP_126336306.1	tail length tape measure protein	-
KGP24_RS23950 (KGP24_23810)	18507..19274	-	768	WP_182403680.1	baseplate	-
KGP24_RS23955 (KGP24_23815)	19271..20065	-	795	WP_223563663.1	phage tail protein	-
KGP24_RS23960 (KGP24_23820)	20208..20519	+	312	WP_079899846.1	type II toxin-antitoxin system HigB family toxin	Toxin
KGP24_RS23965 (KGP24_23825)	20516..20935	+	420	WP_079899847.1	helix-turn-helix domain-containing protein	Antitoxin
KGP24_RS23970 (KGP24_23830)	21006..21743	+	738	WP_182403676.1	hypothetical protein	-
KGP24_RS23975 (KGP24_23835)	21758..22957	-	1200	WP_182403674.1	phage tail protein	-
KGP24_RS23980 (KGP24_23840)	22950..23279	-	330	WP_126336314.1	baseplate protein	-
KGP24_RS23985 (KGP24_23845)	23339..23992	-	654	WP_182403672.1	maturation control protein	-
KGP24_RS23990	24311..25144	+	834	WP_223563664.1	hypothetical protein	-
KGP24_RS23995 (KGP24_23855)	25200..25376	-	177	WP_182403670.1	hypothetical protein	-
KGP24_RS24000	25422..25508	-	87	Protein_28	host cell division inhibitor Icd-like protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..88155	88155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12389.15 Da
• Isoelectric Point: 9.8984

>T201677 WP_079899846.1 NZ_CP074151:20208-20519 [Enterobacter sp. JBIWA008]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMRTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T201677 NZ_CP098714:c954361-954158 [Yersinia ruckeri]
ATGACCAAAACTGACTACCTAATGCGTTTAAGAAAATGCACAACAATTGACACACTAGAGCGTGTTATTGAAAAAAATAA
GTATGAGCTATCTGATGATGAACTAGAATTATTCTATTCTGCTGCGGATCATCGTCTAGCTGAACTTACCATGAATAAGT
TATACGATAAAATTCCTGCAACTGTCTGGCAACATGTGAAGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15395.50 Da
• Isoelectric Point: 4.4420

>AT201677 WP_079899847.1 NZ_CP074151:20516-20935 [Enterobacter sp. JBIWA008]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEMPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT201677 NZ_CP098714:c954781-954413 [Yersinia ruckeri]
ATGGATGAGTACTCACCTAAGCGACATGATATTGCTCAGTTGAAATTCTTATGTGAGAATTTGTATGATGAAGGTATCGC
CACATTGGGCGATAGCCACCACGGCTGGGTTAATGATCCAACCTCCGCGGTAAATCTCCAACTGAATGATTTAATCGAAC
ACATAGCTTCATTTGTGATGAGTTTTAAAATAAAATACGCAGACGAAATCGATCTCTGCGAGTTGGTGGAGGAATATCTA
GACGATACATATACCTTGTTCAGCAGTTACGGAATCAATGATCCTGATTTACGCCGCTGGCAGAAAACAAAAGACCGATT
ATTCAGACTATTTTCAGGGGAATACCTATGTGCCCTGATGAAAAATTAA