Detailed information of TA system TA201675

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3730606..3731263
• Replicon: chromosome
• Accession: NZ_CP074149
• Organism: Enterobacter sp. JBIWA008

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1Z3MZ87
• Locus tag: KGP24_RS17955
• Protein ID: WP_063408809.1
• Coordinates: 3730606..3730905 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KGP24_RS17960
• Protein ID: WP_214577165.1
• Coordinates: 3730916..3731263 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP24_RS17945 (KGP24_17840)	3726977..3729175	+	2199	WP_223561324.1	type I secretion system permease/ATPase	-
KGP24_RS17950 (KGP24_17845)	3729186..3730355	+	1170	WP_086374946.1	HlyD family efflux transporter periplasmic adaptor subunit	-
KGP24_RS17955 (KGP24_17850)	3730606..3730905	+	300	WP_063408809.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KGP24_RS17960 (KGP24_17855)	3730916..3731263	+	348	WP_214577165.1	HigA family addiction module antitoxin	Antitoxin
KGP24_RS17965 (KGP24_17860)	3731308..3731733	+	426	WP_223561325.1	nucleoside triphosphatase NudI	-
KGP24_RS17970 (KGP24_17865)	3731705..3732436	-	732	WP_223561326.1	helix-turn-helix transcriptional regulator	-
KGP24_RS17975 (KGP24_17870)	3732534..3732953	+	420	WP_223561327.1	nuclear transport factor 2 family protein	-
KGP24_RS17980 (KGP24_17875)	3733060..3734241	-	1182	WP_223561328.1	PLP-dependent aminotransferase family protein	-
KGP24_RS17985 (KGP24_17880)	3734262..3735149	-	888	WP_223561329.1	LysR family transcriptional regulator	-
KGP24_RS17990 (KGP24_17885)	3735247..3735849	+	603	WP_223561330.1	short chain dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-71)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11707.32 Da
• Isoelectric Point: 10.0346

>T201675 WP_063408809.1 NZ_CP074149:3730606-3730905 [Enterobacter sp. JBIWA008]
MISYFRDQWLEDFFLYGRSSNVIPSNLETALARKLDIIRAATSHRDLRSPPGNMYEALNPPLKGYSSIRVNRQYRLVFRW
TEGKAEDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T201675 NZ_CP098714:456005-456295 [Yersinia ruckeri]
ATGACCTTTAATATCGACTTTGACGAACGGGCATTAAAAGAATGGCACAAACTAGATAAAACTATCCGAGAGCAATTTAA
AAAGAAACTAAGAAAATTACAGGAAAATCCCTATATTGAGTCAGCACGTTTGCACGGTGATCTGGCTGGTTGTTTCAAAA
TAAAATTACGCGCATCTGGCTTTCGGTTGATTTACCAAGTTATCGATGAGGAGATTGTGATTTTGATCGTTGCTGTTGGT
AAACGGGAAGATGAAAAAGCTTATGATCTTGCCAAGAAGCGAATCCAATAG

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13055.98 Da
• Isoelectric Point: 7.0134

>AT201675 WP_214577165.1 NZ_CP074149:3730916-3731263 [Enterobacter sp. JBIWA008]
MTLQQALRKPTTPGDVLQYEYLEPLNLKISDLAEMLNVHRNTISALVNNNRKLTADMAIKLAKAFDTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVADVMAKRKSGQPDVA

Nucleotide

• Length: 348 bp

>AT201675 NZ_CP098714:455761-456015 [Yersinia ruckeri]
ATGAGTACTATCCAAATTCGTGTGGATGATGAACTGAAGAAAAGTGCATATCAGGCGTTTGAAAAACTGAATCTTTCGCC
TTCTGATGCACTACGCCTGTTCCTACGTTATGTTGCAGAGAACGAAAAGCTGCCTTTTTCAGAAGTATCCGTCATGGTCA
GTAATCAAGATGAGGATGCTGATATTTTGGCTGTTGTCCGTGAACGTTTGAAAAATCCTGCAAAGCGTATCAGAGTAAAT
ATTGATGACCTTTAA

Structures

Toxin

• AlphaFold DB: A0A1Z3MZ87