Detailed information of TA system TA201659

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 5867767..5868419
• Replicon: chromosome
• Accession: NZ_CP074147
• Organism: Serratia sp. JSRIV001

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U2LU32
• Locus tag: KGP17_RS27210
• Protein ID: WP_021181419.1
• Coordinates: 5868078..5868419 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KGP17_RS27205
• Protein ID: WP_021181420.1
• Coordinates: 5867767..5868081 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP17_RS27190 (KGP17_27100)	5863521..5865350	+	1830	WP_115160432.1	glutamine--fructose-6-phosphate transaminase (isomerizing)	-
KGP17_RS27195 (KGP17_27105)	5865473..5866588	+	1116	WP_223498646.1	hypothetical protein	-
KGP17_RS27200 (KGP17_27110)	5866590..5867669	+	1080	WP_223498647.1	GNAT family N-acetyltransferase	-
KGP17_RS27205 (KGP17_27115)	5867767..5868081	-	315	WP_021181420.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
KGP17_RS27210 (KGP17_27120)	5868078..5868419	-	342	WP_021181419.1	hypothetical protein	Toxin
KGP17_RS27215 (KGP17_27125)	5868787..5869827	+	1041	WP_021181417.1	phosphate ABC transporter substrate-binding protein PstS	-
KGP17_RS27220 (KGP17_27130)	5869918..5870874	+	957	WP_024527373.1	phosphate ABC transporter permease PstC	-
KGP17_RS27225 (KGP17_27135)	5870876..5871766	+	891	WP_059199278.1	phosphate ABC transporter permease PstA	-
KGP17_RS27230 (KGP17_27140)	5871814..5872590	+	777	WP_021181414.1	phosphate ABC transporter ATP-binding protein PstB	-
KGP17_RS27235 (KGP17_27145)	5872603..5873334	+	732	WP_024484696.1	phosphate signaling complex protein PhoU	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Antitoxin MqsA/HigA-2 (29-99)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13473.66 Da
• Isoelectric Point: 9.7468

>T201659 WP_021181419.1 NZ_CP074147:c5868419-5868078 [Serratia sp. JSRIV001]
MDAIFVELSIFEAKRADYLSDDEFKTFQQMLLINPFCGDVIQHTGGLRKVRFADHRRGKGKRGGLRVIYYWWLEKSHFLL
FVIYDKDKKDDLSRNEREMLRRMLEILKQGGTL

Nucleotide

• Length: 342 bp

>T201659 NZ_CP098710:471301-471591 [Yersinia ruckeri]
ATGACCTTTAATATCGACTTTGACGAACGGGCATTAAAAGAATGGCACAAACTAGATAAAACTATCCGAGAGCAATTTAA
AAAGAAACTAAGAAAATTACAGGAAAATCCCTATATTGAATCAGCACGTTTGCACGGTGATCTGGCTGGTTGTTTCAAAA
TAAAATTACGCGCATCTGGCTTTCGGTTGATTTACCAAGTTATCGATGAGGAGATTGTGATTTGGATCGTTGCTGTTGGT
AAACGGGAAGATGAAAAAGCTTATGATATTGCCAAGAAGCGAATCCAATAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11870.69 Da
• Isoelectric Point: 7.2018

>AT201659 WP_021181420.1 NZ_CP074147:c5868081-5867767 [Serratia sp. JSRIV001]
MSELSLFEELSQGMKDWSDYNEGKITLKTHAVSLKKNISMSPDELKAIREKLRLSQAVFADYLHTGVTTYQNWEQGRAKP
NKQALLLIRMVEKDPMTLDTLAAL

Nucleotide

• Length: 315 bp

>AT201659 NZ_CP098710:471057-471311 [Yersinia ruckeri]
ATGAGTACTATCCAAATTCGTGTGGATGATGAACTGAAGAAAAGTGCATATCAGGCGTTTGAAAAACTGAATCTTTCGCC
TTCTGATGCACTACGCCTGTTCCTACGTTATGTTGCAGAGAACGAAAAGCTGCCTTTTTCAGAAGTATCCGTCATGGTCA
GTAATCAAGATGAGGATGCTGATATTTTGGCTGTTGTCCGTGAACGTTTGAAAAATCCTGCAAAGCGTATCAGAGTAAAT
ATTGATGACCTTTAA

Structures

Toxin

• AlphaFold DB: U2LU32