Detailed information of TA system TA201623

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3771148..3771807
• Replicon: chromosome
• Accession: NZ_CP074143
• Organism: Serratia sp. JSRIV002

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KGP26_RS17750
• Protein ID: WP_223664350.1
• Coordinates: 3771454..3771807 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: U2NFA4
• Locus tag: KGP26_RS17745
• Protein ID: WP_021180280.1
• Coordinates: 3771148..3771450 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP26_RS17725 (KGP26_17665)	3766475..3767644	+	1170	WP_179250062.1	flavin-dependent monooxygenase	-
KGP26_RS17730 (KGP26_17670)	3767665..3768609	+	945	WP_223664347.1	flavin reductase family protein	-
KGP26_RS17735 (KGP26_17675)	3768682..3769884	-	1203	WP_223664349.1	Bcr/CflA family multidrug efflux MFS transporter	-
KGP26_RS17740 (KGP26_17680)	3770144..3771004	-	861	WP_071680979.1	aldo/keto reductase family oxidoreductase	-
KGP26_RS17745 (KGP26_17685)	3771148..3771450	-	303	WP_021180280.1	XRE family transcriptional regulator	Antitoxin
KGP26_RS17750 (KGP26_17690)	3771454..3771807	-	354	WP_223664350.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KGP26_RS17755 (KGP26_17695)	3772065..3773252	-	1188	WP_223664351.1	sugar transporter	-
KGP26_RS17760 (KGP26_17700)	3773425..3774141	-	717	WP_021805811.1	ABC transporter ATP-binding protein	-
KGP26_RS17765 (KGP26_17705)	3774138..3775013	-	876	WP_024485157.1	ATP-binding cassette domain-containing protein	-
KGP26_RS17770 (KGP26_17710)	3775010..3776296	-	1287	WP_223664352.1	high-affinity branched-chain amino acid ABC transporter permease LivM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13588.58 Da
• Isoelectric Point: 10.1319

>T201623 WP_223664350.1 NZ_CP074143:c3771807-3771454 [Serratia sp. JSRIV002]
VWKIKTTDTFDEWFSRLDDTDRASVLASLIVLREKGPQLPRPYADTVKGSRYSNMKELRVQSRGIPIRAFFAFDPRRRGI
LLCAGSKVGNEKRFYEVMIPIADREFTQFLNTAENKE

Nucleotide

• Length: 354 bp

>T201623 NZ_CP098695:c2999041-2998619 [Yersinia ruckeri]
GTGGCCCAGTGGCGATGTGATCTGCGGGTTTCATGGCGCACCCAACTTTTCTCACTATTGGTACACGGTGTTCTGGTATT
ACTGACACTGGTGGTACCTTGGCCTGACGGATTTACTCCGCTATGGCTTATATTGCTGACGCTGGTGGTGTTTGAATGCA
TTCGCAGCCAGAAGAACATTACCTCTCGACAGGGAGAGATCCGGCTTAAAGCGGGAAATCTGTTGATTTGGAAGTGGCAA
GAGTGGACGTTGGTGCGGCCGCCGTGGATTACCCGCTACGGCGTATTATTATCGTTACAAGGCGCTGGCAGCCGCCGCCG
AAAACGTATTTGGCTAGCCTCCGACAGCATGTCGGAAGAAAAATGGCGCGAGTTGTGTCTGATATTACGACATACTTTTG
ATTCTGCGGCAGGCCGTGAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11022.78 Da
• Isoelectric Point: 6.4749

>AT201623 WP_021180280.1 NZ_CP074143:c3771450-3771148 [Serratia sp. JSRIV002]
MGRTLEQILAEEKPEVVASAERIAADTLLNIHLAELRERVQKTQVEMAQALGIKQPTVAGMEKPGRDLKLSSLKRYIEAA
GGKLRLDIELPDGSHYGFVI

Nucleotide

• Length: 303 bp

>AT201623 NZ_CP098695:c2999288-2999022 [Yersinia ruckeri]
ATGGAAATTGATAACAAAGCCCGAATCCATTGGGCATGCCGCCGGGGTATGCGTGAACTGGATATCTCCATAATGCCATT
TTTCGAACATGAATATGACACGCTAAACGATAATGATAAGCGCGTATTTATCCGCTTGCTGACGAGTGATGATCCTGATT
TATTCAATTGGTTGATGAACCACGGCGAGCCGCAGGATAGCGAACTGAAACGAATGGTTAAACTGATTCAGACACGAAAT
AAAGCCCGTGGCCCAGTGGCGATGTGA

Structures

Antitoxin

• AlphaFold DB: U2NFA4