Detailed information of TA system TA201609

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 742616..743272
• Replicon: chromosome
• Accession: NZ_CP074143
• Organism: Serratia sp. JSRIV002

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7Y8XPF0
• Locus tag: KGP26_RS03350
• Protein ID: WP_039995466.1
• Coordinates: 742949..743272 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KGP26_RS03345
• Protein ID: WP_223665015.1
• Coordinates: 742616..742906 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP26_RS03320 (KGP26_03315)	738289..739017	-	729	WP_223665012.1	RES family NAD+ phosphorylase	-
KGP26_RS03325 (KGP26_03320)	738998..739759	-	762	WP_051363780.1	hypothetical protein	-
KGP26_RS03330 (KGP26_03325)	739885..740706	-	822	WP_223665013.1	helix-turn-helix transcriptional regulator	-
KGP26_RS03335 (KGP26_03330)	740850..741362	+	513	WP_021807989.1	helix-turn-helix domain-containing protein	-
KGP26_RS03340 (KGP26_03335)	741643..742563	+	921	WP_223665014.1	L,D-transpeptidase family protein	-
KGP26_RS03345 (KGP26_03340)	742616..742906	-	291	WP_223665015.1	NadS family protein	Antitoxin
KGP26_RS03350 (KGP26_03345)	742949..743272	-	324	WP_039995466.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KGP26_RS03355 (KGP26_03350)	743491..744207	-	717	WP_223665016.1	GntR family transcriptional regulator	-
KGP26_RS03360 (KGP26_03355)	744442..746367	+	1926	WP_223665017.1	PTS 2-O-a-mannosyl-D-glycerate transporter subunit IIABC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (45-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-80)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12404.25 Da
• Isoelectric Point: 9.2975

>T201609 WP_039995466.1 NZ_CP074143:c743272-742949 [Serratia sp. JSRIV002]
MEYFEFIETPVFSRQRETLLDDDSFRELQTHLLKYHSDGDTISKTGGCKKIRWNRPGMGKQGGVRVIYYVITRSGKLYLL
IVYPKNAKDDLTESEKAILKALTDKLN

Nucleotide

• Length: 324 bp

>T201609 NZ_CP098694:546586-546927 [Yersinia ruckeri]
ATGAAAACTCTACCTGCTACAATTTCGCGGGCGACGAAGCCCTGTCTGTCGCCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACAACACGCCGTTCAGTGATGAAACTGTTATTCAGGAACACATAG
ACGCCGGTATCACGCTGGCTGATGCCGTTAATTTTCTGGTGGAAAAATATGAACTGGTTCGTATTGATCGAAGAGGATTT
AGTTGGCAGGAACAATCACCTTATCTTCGGGCTGTAGATATCCTGCGAGCACGACAAGCAACTAGCTTGTTGCGACGGAG
CCATAATAACGCTGTATTGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10884.57 Da
• Isoelectric Point: 6.4836

>AT201609 WP_223665015.1 NZ_CP074143:c742906-742616 [Serratia sp. JSRIV002]
VDKELFNDLVESMEQMVAIEKGEMQPARVHRHHIPNVKSLREASGMKQEEFAQAVGVSPSLVQSWEQNRRIPSGSSLKIL
LMIERHPTIINDLLAL

Nucleotide

• Length: 291 bp

>AT201609 NZ_CP098694:546245-546565 [Yersinia ruckeri]
ATGAGAAGTAACGTCACATGGGGCCTGCAGCGGGATACCACGCCGTGTCTGGGAGCCCGTCTGGTACAGGAGGGGAACCA
ACGGCATTATCTGGCTGACCGGGCCAGTATTACCGGTAAATTCAGTGACACCGAATGCCGGAAGCTGGATGAAACATTCC
CGCACTTTATCAGCCAGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGCGTCATCCTGTAT
CACAACGGTTTCACCTGCGAAGCCGACACGCTTGGCAGTTGCGGCTATGTATACATCGCCGTCTATCCCACTCAACGCTA
A

Structures

Toxin

• AlphaFold DB: A0A7Y8XPF0