Detailed information of TA system TA201565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3582545..3583205
• Replicon: chromosome
• Accession: NZ_CP074137
• Organism: Serratia sp. JSRIV006

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KGP16_RS16635
• Protein ID: WP_218519157.1
• Coordinates: 3582852..3583205 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: U2NFA4
• Locus tag: KGP16_RS16630
• Protein ID: WP_021180280.1
• Coordinates: 3582545..3582847 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP16_RS16610 (KGP16_16565)	3577872..3579041	+	1170	WP_059201170.1	flavin-dependent monooxygenase	-
KGP16_RS16615 (KGP16_16570)	3579062..3580006	+	945	WP_071680978.1	flavin reductase family protein	-
KGP16_RS16620 (KGP16_16575)	3580079..3581281	-	1203	WP_094982866.1	Bcr/CflA family multidrug efflux MFS transporter	-
KGP16_RS16625 (KGP16_16580)	3581541..3582401	-	861	WP_218519158.1	aldo/keto reductase family oxidoreductase	-
KGP16_RS16630 (KGP16_16585)	3582545..3582847	-	303	WP_021180280.1	XRE family transcriptional regulator	Antitoxin
KGP16_RS16635 (KGP16_16590)	3582852..3583205	-	354	WP_218519157.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KGP16_RS16640 (KGP16_16595)	3583463..3584650	-	1188	WP_021805810.1	sugar transporter	-
KGP16_RS16645 (KGP16_16600)	3584824..3585540	-	717	WP_223500005.1	ABC transporter ATP-binding protein	-
KGP16_RS16650 (KGP16_16605)	3585537..3586412	-	876	WP_223500006.1	ATP-binding cassette domain-containing protein	-
KGP16_RS16655 (KGP16_16610)	3586409..3587695	-	1287	WP_223500007.1	high-affinity branched-chain amino acid ABC transporter permease LivM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13602.52 Da
• Isoelectric Point: 9.5694

>T201565 WP_218519157.1 NZ_CP074137:c3583205-3582852 [Serratia sp. JSRIV006]
VWEIKTTDTFDEWFSRLEDTDRASVLASLIVLREKGPQLPRPYADTVKGSRYSNMKELRVQSRGIPIRAFFAFDPRRRGI
LLCAGNKAGNEKRFYEVMIPIADREFTQFLNTAENKE

Nucleotide

• Length: 354 bp

>T201565 NZ_CP098538:c1580080-1579898 [Neisseria gonorrhoeae]
TTGAATAGCCTAGACGTTATTGCCCTACTCAAACAAGACGGTTGGTATAAAGTTGCACAATCCGGGAGCCATTCGCAATA
TAAGCACCCAACAAAAAAAGGCCGTGTAACCGTGCCGCACCCGAAAAAAGACTTGCCGACAGGTACTGTAAAAAATATCT
ATAAGCAAGCCGGTTTGAAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11022.78 Da
• Isoelectric Point: 6.4749

>AT201565 WP_021180280.1 NZ_CP074137:c3582847-3582545 [Serratia sp. JSRIV006]
MGRTLEQILAEEKPEVVASAERIAADTLLNIHLAELRERVQKTQVEMAQALGIKQPTVAGMEKPGRDLKLSSLKRYIEAA
GGKLRLDIELPDGSHYGFVI

Nucleotide

• Length: 303 bp

>AT201565 NZ_CP098538:c1579796-1579395 [Neisseria gonorrhoeae]
ATGTTTATCCCTGCCGCCCTGCACAAAGACGAACATTCGGCATATGGCGTAACCATCCCCGACCTTCCCGGCTGTTTCTC
TTGCGGCGACACCGTTGAAGAAGCCGTCGCCAACGCCCGTTCTGCCGCCTATATGCATATTGACGGTATGATTGAGGACG
GAGGGTTCAAAAACCTTGCCGTAAGCAGCATTGCCGATTTGAGCCAAGAACCTGATTATCATGGGGCAACATGGGTAATG
ATTGAAATCGACCCTGCAAAAATCAGTCGGCAGCAAATCCGATTCAACGTCAGCTGGCCGCAATACCTTCTTGATAGAGT
GGATGAATATACATCCGCAAATCATGAAACCCGTAGCGGTTTTTTAGCCAAAGCCGCCCTGCTTACCATGAATCAGGCAT
AG

Structures

Antitoxin

• AlphaFold DB: U2NFA4