Detailed information of TA system TA201543

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 98730..98983
• Replicon: plasmid pEC16-CTX-55
• Accession: NZ_CP074124
• Organism: Escherichia coli strain EC16

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: KGQ53_RS25600
• Protein ID: WP_001312851.1
• Coordinates: 98834..98983 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	98730..98789 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGQ53_RS25575 (95457)	95457..96203	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
KGQ53_RS25580 (96258)	96258..96818	+	561	WP_000139328.1	fertility inhibition protein FinO	-
KGQ53_RS25585 (96950)	96950..97150	+	201	WP_015059022.1	hypothetical protein	-
KGQ53_RS25590 (97536)	97536..98135	+	600	WP_032083981.1	PIN domain-containing protein	-
KGQ53_RS25595 (98197)	98197..98529	+	333	WP_152916585.1	hypothetical protein	-
- (98730)	98730..98789	-	60	NuclAT_1	-	Antitoxin
- (98730)	98730..98789	-	60	NuclAT_1	-	Antitoxin
- (98730)	98730..98789	-	60	NuclAT_1	-	Antitoxin
- (98730)	98730..98789	-	60	NuclAT_1	-	Antitoxin
KGQ53_RS25600 (98834)	98834..98983	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
KGQ53_RS25605 (99267)	99267..99515	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qnrS1 / fosA3 / blaTEM-1B / blaCTX-M-55	-	1..99826	99826

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T201543 WP_001312851.1 NZ_CP074124:98834-98983 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T201543 NZ_CP098504:c3825-3547 [Neisseria gonorrhoeae]
ATGTACGCGATTTCTTTTGATTTGGTGGTTGCCGATACCGCTCAAAACCATCCGAAAGGCATTTCCCAAGCCTACGCGGA
TATTGGATACACCCTGCGGAAATTCGGCTTTACCCGCATTCAGGGAAGTTTGTACACCTGCCAAAACGAAGATATGGCTA
ACCTGTTTTCAGCCATCAACGAGCTAAAAGCTCTGCCTTGGTTTCCGTCTTCTGTCCGCGATATTCGGGCGTTCCGCATT
GAGCAGTGGTCTGATTTCACAAGTCTTGTGAAGTCTTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT201543 NZ_CP074124:c98789-98730 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin