Detailed information of TA system TA201540

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 59244..59513
• Replicon: plasmid pEC16-CTX-55
• Accession: NZ_CP074124
• Organism: Escherichia coli strain EC16

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KGQ53_RS25370
• Protein ID: WP_001372321.1
• Coordinates: 59388..59513 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	59244..59309 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGQ53_RS25340	54975..55502	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
KGQ53_RS25345	55560..55793	+	234	WP_000006003.1	DUF905 family protein	-
KGQ53_RS25350	55854..57856	+	2003	Protein_66	ParB/RepB/Spo0J family partition protein	-
KGQ53_RS25355	57925..58359	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
KGQ53_RS25360	58356..59118	+	763	Protein_68	plasmid SOS inhibition protein A	-
-	59087..59311	+	225	NuclAT_0	-	-
-	59087..59311	+	225	NuclAT_0	-	-
-	59087..59311	+	225	NuclAT_0	-	-
-	59087..59311	+	225	NuclAT_0	-	-
KGQ53_RS25365	59096..59275	-	180	WP_001309233.1	hypothetical protein	-
-	59244..59309	-	66	-	-	Antitoxin
KGQ53_RS26030	59297..59446	+	150	Protein_70	plasmid maintenance protein Mok	-
KGQ53_RS25370	59388..59513	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
KGQ53_RS25375	59832..60128	-	297	Protein_72	hypothetical protein	-
KGQ53_RS25380	60428..60724	+	297	WP_001272251.1	hypothetical protein	-
KGQ53_RS25385	60835..61656	+	822	WP_212639660.1	DUF932 domain-containing protein	-
KGQ53_RS25390	61953..62600	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
KGQ53_RS25395	62877..63260	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KGQ53_RS25400	63451..64137	+	687	WP_212639661.1	PAS domain-containing protein	-
KGQ53_RS25405	64230..64457	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qnrS1 / fosA3 / blaTEM-1B / blaCTX-M-55	-	1..99826	99826

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T201540 WP_001372321.1 NZ_CP074124:59388-59513 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T201540 NZ_CP098502:2586198-2586497 [Paraconexibacter antarcticus]
ATGCGCCCCATCCATATCGCGCGGCTCGACAAGACCCGCCCGGTCCTGGTGCTCACCCGCGAACTGGTGCGCGCTCACCT
GAGGTCGGTCACCGTCGCACCGATCACCACCACGATCCGCGGCCTCTCCACCGAGGTCCCGGTCGGGCCGCCCAACGGCC
TCTCCGGCCCGTCGGTGGTGAGCTGCGACAACATCACAACGATCCCCGTCGCGGCGCTTGGTCAGCAGACTGGTGTCCTG
CTCGACCGCCAGGAACCAGCCCTCGCCGAGGCAATCGCGATGGCCTTCGACCTGGAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT201540 NZ_CP074124:c59309-59244 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin