Detailed information of TA system TA201530

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 117501..117927
• Replicon: plasmid pEC16-NDM-5
• Accession: NZ_CP074121
• Organism: Escherichia coli strain EC16

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KGQ53_RS23680
• Protein ID: WP_001372321.1
• Coordinates: 117501..117626 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	117703..117927 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGQ53_RS23655 (112875)	112875..113564	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
KGQ53_RS23660 (113751)	113751..114134	-	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KGQ53_RS23665 (114467)	114467..115057	+	591	WP_001376243.1	transglycosylase SLT domain-containing protein	-
KGQ53_RS23670 (115354)	115354..116175	-	822	WP_001234426.1	DUF932 domain-containing protein	-
KGQ53_RS23675 (116293)	116293..116580	-	288	WP_000107535.1	hypothetical protein	-
KGQ53_RS25950 (116605)	116605..116811	-	207	WP_000547968.1	hypothetical protein	-
KGQ53_RS25955 (116881)	116881..117053	+	173	Protein_125	hypothetical protein	-
KGQ53_RS25960 (117051)	117051..117281	-	231	WP_071586998.1	hypothetical protein	-
KGQ53_RS23680 (117501)	117501..117626	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
KGQ53_RS25965 (117568)	117568..117717	-	150	Protein_128	plasmid maintenance protein Mok	-
- (117703)	117703..117927	-	225	NuclAT_0	-	Antitoxin
- (117703)	117703..117927	-	225	NuclAT_0	-	Antitoxin
- (117703)	117703..117927	-	225	NuclAT_0	-	Antitoxin
- (117703)	117703..117927	-	225	NuclAT_0	-	Antitoxin
KGQ53_RS23685 (117739)	117739..117927	+	189	WP_001299721.1	hypothetical protein	-
KGQ53_RS23690 (117896)	117896..118658	-	763	Protein_130	plasmid SOS inhibition protein A	-
KGQ53_RS23695 (118655)	118655..119089	-	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
KGQ53_RS23700 (119144)	119144..119341	-	198	Protein_132	hypothetical protein	-
KGQ53_RS23705 (119369)	119369..119602	-	234	WP_000005987.1	DUF905 family protein	-
KGQ53_RS23710 (119670)	119670..120167	-	498	WP_042039995.1	single-stranded DNA-binding protein	-
KGQ53_RS23715 (120317)	120317..122218	-	1902	WP_000936285.1	group II intron reverse transcriptase/maturase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / aac(3)-IVa / aph(4)-Ia / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..145550	145550

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T201530 WP_001372321.1 NZ_CP074121:c117626-117501 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T201530 NZ_CP098486:c3768047-3767637 [Enterobacter hormaechei subsp. hormaechei]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGTCTGGTCGCGGC
AATGGTGTTATTGGTGCCGTGGCCGCTAAGCTATACCCCTCTGTGGCTGCTTTTGCTGTCGTTTGTAGTGTTTGACAGCG
TGCGCAGCCAGCGTCGAATTAACGCCCGTCAGGGTGAAATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAAATGATAGGGACGCCGTGGATGCTCAACACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGACAATCGTCG
CCAGCACCTGTGGCTGGCAGCAGACAGTATGGATCCTGCGGAGTGGCGAGATCTGCGCCGGTTGATCGTGCAACAGCCGA
CGCAGGAGTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT201530 NZ_CP074121:c117927-117703 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin