Detailed information of TA system TA201525

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 79172..79426
• Replicon: plasmid pEC16-NDM-5
• Accession: NZ_CP074121
• Organism: Escherichia coli strain EC16

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: KGQ53_RS23460
• Protein ID: WP_001312851.1
• Coordinates: 79172..79321 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	79365..79426 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGQ53_RS23425 (74724)	74724..75125	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
KGQ53_RS23430 (75058)	75058..75315	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
KGQ53_RS23435 (75408)	75408..76061	-	654	WP_000616800.1	type II CAAX endopeptidase family protein	-
KGQ53_RS23440 (77000)	77000..77857	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
KGQ53_RS23445 (77850)	77850..78332	-	483	WP_001273588.1	hypothetical protein	-
KGQ53_RS25940 (78325)	78325..78372	-	48	WP_229471593.1	hypothetical protein	-
KGQ53_RS25945 (78363)	78363..78614	+	252	WP_223195197.1	replication protein RepA	-
KGQ53_RS23455 (78631)	78631..78888	-	258	WP_000083845.1	replication regulatory protein RepA	-
KGQ53_RS23460 (79172)	79172..79321	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (79365)	79365..79426	+	62	NuclAT_1	-	Antitoxin
- (79365)	79365..79426	+	62	NuclAT_1	-	Antitoxin
- (79365)	79365..79426	+	62	NuclAT_1	-	Antitoxin
- (79365)	79365..79426	+	62	NuclAT_1	-	Antitoxin
KGQ53_RS23465 (79682)	79682..79756	-	75	Protein_81	endonuclease	-
KGQ53_RS23470 (80002)	80002..80214	-	213	WP_001299730.1	ANR family transcriptional regulator	-
KGQ53_RS23475 (80350)	80350..80910	-	561	WP_000139315.1	fertility inhibition protein FinO	-
KGQ53_RS23480 (81013)	81013..81873	-	861	WP_000704514.1	alpha/beta hydrolase	-
KGQ53_RS23485 (81932)	81932..82678	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / aac(3)-IVa / aph(4)-Ia / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..145550	145550

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T201525 WP_001312851.1 NZ_CP074121:c79321-79172 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T201525 NZ_CP098486:30480-30794 [Enterobacter hormaechei subsp. hormaechei]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTAAGCCAGTTCCCGCAATACAGAGAAGAGCTTCTCTTTTTAGG
ACGAGTTATTGAAAAGAGCCACTGCCCGACGCCCGCTGCACTGAGAAAACTTTTCCCAACGCTGGATAACTTCAAGTATC
TTGATAAGCATTATGTTATTGATATTGCAAATAACAATCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT201525 NZ_CP074121:79365-79426 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin