Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3707055..3707782 Replicon chromosome
Accession NZ_CP074120
Organism Escherichia coli strain EC16

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag KGQ53_RS17740 Protein ID WP_000550189.1
Coordinates 3707055..3707369 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KGQ53_RS17745 Protein ID WP_000560266.1
Coordinates 3707366..3707782 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KGQ53_RS17720 (3703213) 3703213..3704199 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
KGQ53_RS17725 (3704278) 3704278..3704970 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
KGQ53_RS17730 (3705047) 3705047..3705550 - 504 WP_001333820.1 M48 family metallopeptidase -
KGQ53_RS17735 (3705635) 3705635..3706771 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
KGQ53_RS17740 (3707055) 3707055..3707369 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
KGQ53_RS17745 (3707366) 3707366..3707782 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
KGQ53_RS17750 (3707827) 3707827..3709845 - 2019 WP_000121435.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
KGQ53_RS17755 (3710271) 3710271..3712622 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T201520 WP_000550189.1 NZ_CP074120:3707055-3707369 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T201520 NZ_CP098480:c2555573-2555184 [Acinetobacter sp. C32I]
ATGGCTAATCCTGCTTTTGAGTTGCAATACAGTCGTTTTGCGTTTGTACTGCAACTCTTACTGTTTATTCTGATTCTTAG
TGTACTTTATCCCTTACTCCCCCTTTGGTGGTGGTTACTGAGCTTTATGTTGATGACCATCGCGTGGTTATCGTTTTTGC
GGCAACCCCAAATTAAACGTTTTGAATACTTAGATCATCAAGACTGTTCATTTGAATTTTCTGATCCTACCTTGAAAATT
CAGCGTAGGCAGATCGTCAAAATATTAGATCACCAACTCTATATTGCCCTCTATTTTTCTCAGCAAAAGCATAAAACTTG
CATTATTTGGTGGGATCAATTGTCACATGCACAGTGGAAAAAGCTAAAATTACGCGCAAAACTAGCTTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT201520 WP_000560266.1 NZ_CP074120:3707366-3707782 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT201520 NZ_CP098480:c2555823-2555566 [Acinetobacter sp. C32I]
ATGTCTGATGAAATGACACTGGAAGAGCGTAAGGTTGTATATCGTGCCCGTCGTGGTTTAAAAGAAATCGATGTGTATTT
CGACCCGTACGTTAAAAATTACTATTTAACTGCTGATGCTGATGAAAAAGCCTTATTTGCCGAGCTCGTCGATCAAGAAG
ACCCAGATTTATTGGATTGGTTTATGGAAGTGGGTGAGCCACCGCGTATTGAGTTAAAAGAGTTTATTCTCAAACTCAAA
CATTATGTGCATGGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References