Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1990517..1991211 Replicon chromosome
Accession NZ_CP074120
Organism Escherichia coli strain EC16

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag KGQ53_RS09595 Protein ID WP_001263489.1
Coordinates 1990517..1990915 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag KGQ53_RS09600 Protein ID WP_000554758.1
Coordinates 1990918..1991211 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (1986105) 1986105..1986185 - 81 NuclAT_12 - -
- (1986105) 1986105..1986185 - 81 NuclAT_12 - -
- (1986105) 1986105..1986185 - 81 NuclAT_12 - -
- (1986105) 1986105..1986185 - 81 NuclAT_12 - -
KGQ53_RS09570 (1986781) 1986781..1987239 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
KGQ53_RS09575 (1987500) 1987500..1988957 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
KGQ53_RS09580 (1989014) 1989014..1989535 - 522 Protein_1901 peptide chain release factor H -
KGQ53_RS09585 (1989531) 1989531..1989737 - 207 Protein_1902 RtcB family protein -
KGQ53_RS09590 (1990055) 1990055..1990507 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
KGQ53_RS09595 (1990517) 1990517..1990915 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
KGQ53_RS09600 (1990918) 1990918..1991211 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
KGQ53_RS09605 (1991263) 1991263..1992318 - 1056 WP_001226164.1 DNA polymerase IV -
KGQ53_RS09610 (1992389) 1992389..1993174 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
KGQ53_RS09615 (1993146) 1993146..1994858 + 1713 Protein_1908 flagellar biosynthesis protein FlhA -
KGQ53_RS09620 (1995082) 1995082..1995579 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T201514 WP_001263489.1 NZ_CP074120:c1990915-1990517 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T201514 NZ_CP098473:c1286-1008 [Neisseria gonorrhoeae]
ATGTACGCGATTTCTTTTGATTTGGTGGTTGCCGATACCGCTCAAAACCATCCGAAAGGCATTTCCCAAGCCTACGCGGA
TATTGGATACACCCTGCGGAAATTCGGCTTTACCCGCATTCAGGGAAGTTTGTACACCTGCCAAAACGAAGATATGGCTA
ACCTGTTTTCAGCCATCAACGAGCTAAAAGCTCTGCCTTGGTTTCCGTCTTCTGTCCGCGATATTCGGGCGTTCCGCATT
GAGCAGTGGTCTGATTTCACAAGTCTTGTGAAGTCTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT201514 WP_000554758.1 NZ_CP074120:c1991211-1990918 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT201514 NZ_CP098473:c1471-1286 [Neisseria gonorrhoeae]
ATGCAAACCACTATCCCCACCGGTTCAATCCGCACCTTTGGCGATTACGGCGTGATGTACATCGTCGGCACGCCTGCCGA
ACAGCTTCCGGACGGCGATTGGCTCGTAAATATTGAACTGCCGGAAAGCGGCGAACACACGCAATACAAACTGTCCCACA
TCGTTCAAGACCCAAAGGCTGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References