Detailed information of TA system TA201501

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 104229..104731
• Replicon: chromosome
• Accession: NZ_CP074120
• Organism: Escherichia coli strain EC16

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: KGQ53_RS00515
• Protein ID: WP_000767829.1
• Coordinates: 104477..104731 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: KGQ53_RS00510
• Protein ID: WP_001259255.1
• Coordinates: 104229..104480 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGQ53_RS00485 (99406)	99406..100473	-	1068	WP_000080090.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
KGQ53_RS00490 (100473)	100473..101543	-	1071	WP_000108933.1	histidinol-phosphate transaminase	-
KGQ53_RS00495 (101540)	101540..102844	-	1305	WP_074514295.1	histidinol dehydrogenase	-
KGQ53_RS00500 (102850)	102850..103749	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
KGQ53_RS00505 (103895)	103895..103945	-	51	WP_001364200.1	his operon leader peptide	-
KGQ53_RS00510 (104229)	104229..104480	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
KGQ53_RS00515 (104477)	104477..104731	+	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
KGQ53_RS00520 (104814)	104814..105638	+	825	WP_000754737.1	SDR family oxidoreductase	-
KGQ53_RS00525 (105684)	105684..106613	+	930	WP_000803366.1	LysR substrate-binding domain-containing protein	-
KGQ53_RS00530 (106828)	106828..106890	+	63	WP_010723108.1	membrane protein YoeI	-
KGQ53_RS00535 (106880)	106880..108238	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
KGQ53_RS00540 (108417)	108417..109475	+	1059	WP_087523589.1	thiosulfate utilization transporter TsuA/YeeE	-
KGQ53_RS00545 (109489)	109489..109716	+	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T201501 WP_000767829.1 NZ_CP074120:104477-104731 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T201501 NZ_CP098464:c1635393-1635211 [Neisseria gonorrhoeae]
TTGAATAGCCTAGACGTTATTGCCCTACTCAAACAAGACGGTTGGTATAAAGTTGCACAATCCGGGAGTCATTCGCAATA
TAAGCACCCAACAAAAAAAGGCCGTGTAACCGTGCCGCACCCGAAAAAAGACTTGCCGACAGGTACTGTAAAAAATATCT
ATAAGCAAGCCGGTTTGAAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT201501 WP_001259255.1 NZ_CP074120:104229-104480 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT201501 NZ_CP098464:c1635109-1634708 [Neisseria gonorrhoeae]
ATGTTTATCCCTGCCGCCCTGCACAAAGACGAACATTCGGCATATGGCGTAACCATCCCCGACCTTCCCGGCTGTTTCTC
TTGCGGCGACACCGTTGAAGAAGCCGTCGCCAACGCCCGTTCTGCCGCCTATATGCATATTGACGGTATGATTGAGGACG
GAGGGTTCAAAAACCTTGCCGTAAGCAGCATTGCCGATTTGAGCCAAGAACCTGATTATCATGGGGCAACATGGGTAATG
ATTGAAATCGACCCTGCAAAAATCAGTCGGCAGCAAATCCGATTCAACGTCAGCTGGCCGCAATACCTTCTTGATAGAGT
GGATGAATATACATCCGCAAATCATGAAACCCGTAGCGGTTTTTTAGCCAAAGCCGCCCTGCTCACCATGAATCAGGCAT
AG

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0