Detailed information of TA system TA201496

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 7363..7616
• Replicon: plasmid unnamed2
• Accession: NZ_CP074118
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain kpn-hnqyy

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: KGB32_RS27870
• Protein ID: WP_001312851.1
• Coordinates: 7363..7512 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	7557..7616 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGB32_RS27835 (2722)	2722..3137	-	416	Protein_4	IS1-like element IS1B family transposase	-
KGB32_RS27840 (3386)	3386..3787	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
KGB32_RS27845 (3720)	3720..3977	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
KGB32_RS27850 (4070)	4070..4723	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
KGB32_RS27855 (5662)	5662..6519	-	858	WP_142295464.1	incFII family plasmid replication initiator RepA	-
KGB32_RS28870 (6538)	6538..6716	-	179	Protein_9	protein CopA/IncA	-
KGB32_RS27865 (6831)	6831..7079	-	249	WP_000083837.1	replication regulatory protein RepA	-
KGB32_RS27870 (7363)	7363..7512	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (7557)	7557..7616	+	60	NuclAT_0	-	Antitoxin
- (7557)	7557..7616	+	60	NuclAT_0	-	Antitoxin
- (7557)	7557..7616	+	60	NuclAT_0	-	Antitoxin
- (7557)	7557..7616	+	60	NuclAT_0	-	Antitoxin
KGB32_RS27875 (7817)	7817..8149	-	333	WP_152916585.1	hypothetical protein	-
KGB32_RS27880 (8211)	8211..8810	-	600	WP_032083981.1	PIN domain-containing protein	-
KGB32_RS27885 (9196)	9196..9396	-	201	WP_015059022.1	hypothetical protein	-
KGB32_RS27890 (9528)	9528..10088	-	561	WP_000139328.1	fertility inhibition protein FinO	-
KGB32_RS27895 (10143)	10143..10889	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
KGB32_RS27900 (10909)	10909..11109	-	201	WP_072354025.1	hypothetical protein	-
KGB32_RS27905 (11134)	11134..11838	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
KGB32_RS27910 (11844)	11844..11984	+	141	WP_001044210.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / blaKPC-2	-	1..116047	116047

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T201496 WP_001312851.1 NZ_CP074118:c7512-7363 [Klebsiella pneumoniae subsp. pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T201496 NZ_CP098461:3789-4067 [Neisseria gonorrhoeae]
ATGTACGCGATTTCTTTTGATTTGGTGGTTGCCGATACCGCTCAAAACCATCCGAAAGGCATTTCCCAAGCCTACGCGGA
TATTGGATACACCCTGCGGAAATTCGGCTTTACCCGCATTCAGGGAAGTTTGTACACCTGCCAAAACGAAGATATGGCTA
ACCTGTTTTCAGCCATCAACGAGCTAAAAGCTCTGCCTTGGTTTCCGTCTTCTGTCCGCGATATTCGGGCGTTCCGCATT
GAGCAGTGGTCTGATTTCACAAGTCTTGTGAAGTCTTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT201496 NZ_CP074118:7557-7616 [Klebsiella pneumoniae subsp. pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin