Detailed information of TA system TA201493

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 56899..57325
• Replicon: plasmid unnamed1
• Accession: NZ_CP074117
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain kpn-hnqyy

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KGB32_RS27425
• Protein ID: WP_001372321.1
• Coordinates: 56899..57024 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	57101..57325 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGB32_RS27390 (51954)	51954..52181	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
KGB32_RS27395 (52275)	52275..52961	-	687	WP_015059009.1	PAS domain-containing protein	-
KGB32_RS27400 (53152)	53152..53535	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KGB32_RS27405 (53812)	53812..54459	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
KGB32_RS27410 (54756)	54756..55577	-	822	WP_001234445.1	DUF932 domain-containing protein	-
KGB32_RS27415 (55688)	55688..55984	-	297	WP_001272251.1	hypothetical protein	-
KGB32_RS27420 (56284)	56284..56580	+	297	Protein_44	hypothetical protein	-
KGB32_RS27425 (56899)	56899..57024	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
KGB32_RS28815 (56966)	56966..57115	-	150	Protein_46	plasmid maintenance protein Mok	-
- (57103)	57103..57168	+	66	NuclAT_0	-	-
- (57103)	57103..57168	+	66	NuclAT_0	-	-
- (57103)	57103..57168	-	66	NuclAT_1	-	-
- (57101)	57101..57325	+	225	NuclAT_0	-	-
- (57101)	57101..57325	-	225	NuclAT_0	-	Antitoxin
- (57101)	57101..57325	-	225	NuclAT_0	-	Antitoxin
- (57101)	57101..57325	-	225	NuclAT_0	-	Antitoxin
- (57101)	57101..57325	-	225	NuclAT_0	-	Antitoxin
KGB32_RS27430 (57337)	57337..58056	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
KGB32_RS27435 (58053)	58053..58487	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
KGB32_RS27440 (58556)	58556..60579	-	2024	Protein_49	ParB/RepB/Spo0J family partition protein	-
KGB32_RS27445 (60640)	60640..60873	-	234	WP_000006003.1	DUF905 family protein	-
KGB32_RS27450 (60931)	60931..61458	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
KGB32_RS28820 (61760)	61760..62221	+	462	Protein_52	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T201493 WP_001372321.1 NZ_CP074117:c57024-56899 [Klebsiella pneumoniae subsp. pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T201493 NZ_CP098458:c1663771-1663589 [Neisseria gonorrhoeae]
TTGAATAGCCTAGACGTTATTGCCCTACTCAAACAAGACGGTTGGTATAAAGTTGCACAATCCGGGAGCCATTCGCAATA
TAAGCACCCAACAAAAAAAGGCCGTGTAACCGTGCCGCACCCGAAAAAAGACTTGCCGACAGGTACTGTAAAAAATATCT
ATAAGCAAGCCGGTTTGAAGTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT201493 NZ_CP074117:c57325-57101 [Klebsiella pneumoniae subsp. pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin