Detailed information of TA system TA201472

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 2674586..2675181
• Replicon: chromosome
• Accession: NZ_CP074112
• Organism: Idiomarina loihiensis strain SN11

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KGF88_RS12840
• Protein ID: WP_153827326.1
• Coordinates: 2674858..2675181 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KGF88_RS12835
• Protein ID: WP_110075591.1
• Coordinates: 2674586..2674861 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGF88_RS12805 (KGF88_12850)	2669964..2670686	-	723	WP_257119940.1	methyltransferase domain-containing protein	-
KGF88_RS12810 (KGF88_12855)	2671015..2671755	+	741	WP_153827322.1	GPP34 family phosphoprotein	-
KGF88_RS12815 (KGF88_12860)	2671828..2672310	+	483	WP_153827323.1	diguanylate cyclase	-
KGF88_RS12820 (KGF88_12865)	2672297..2673550	-	1254	WP_153827324.1	type II toxin-antitoxin system HipA family toxin	-
KGF88_RS12825 (KGF88_12870)	2673547..2673852	-	306	WP_110075600.1	transcriptional regulator	-
KGF88_RS12830 (KGF88_12875)	2674008..2674589	+	582	WP_153827325.1	SOS response-associated peptidase	-
KGF88_RS12835 (KGF88_12880)	2674586..2674861	-	276	WP_110075591.1	helix-turn-helix transcriptional regulator	Antitoxin
KGF88_RS12840 (KGF88_12885)	2674858..2675181	-	324	WP_153827326.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KGF88_RS12845 (KGF88_12890)	2675443..2676585	+	1143	WP_110077114.1	RNA-guided endonuclease TnpB family protein	-
KGF88_RS12850 (KGF88_12895)	2676650..2677426	-	777	WP_153827300.1	SDR family oxidoreductase	-
KGF88_RS12855 (KGF88_12900)	2677540..2678088	+	549	WP_153827299.1	hypothetical protein	-
KGF88_RS12860 (KGF88_12905)	2678130..2679923	+	1794	WP_153827298.1	HEAT repeat domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2651944..2692251	40307
	-	flank	IS/Tn	-	-	2675443..2676585	1142

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (15-101)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-85)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12010.12 Da
• Isoelectric Point: 9.6825

>T201472 WP_153827326.1 NZ_CP074112:c2675181-2674858 [Idiomarina loihiensis]
MSWTITFYSGVEEGLLSLPPKIQARMIRLLELMETHGPNLGPPHTKSIGNGLFELRAKAQEGIARGLFCYLDGERICVLS
VFVKKTQKIPQNEIEIARKRLAEVKTS

Nucleotide

• Length: 324 bp

>T201472 NZ_CP098446:3721993-3722379 [Proteus mirabilis]
ATGGTTATTTATAAAACAAAGATGTTCAAAAATAGTTTCAAAAAAATAACGCTTACGAATGCAGAACTAATCGCTATCGC
TACCGAAGTCCTTGCAGGACAATTTGAGGCAGATCTAGGTGGTGGTGTTATCAAGAAACGTGCCTGTATCCAAGGAAAAG
GTAAAAGTAGCGGAATTAGAACAATTATATTTTATAAGCAAGGGAATAATTTATTCTTTGCTGATGGTTGGAAAAAATCG
AGTTTAAGCTCTAAAAAAACCAAAGAGATCACAGATGATGAACTTGAGTCCTATAAAGATCTAGCAAAAGATCTCTTCAA
TGCAAATCAAAATAAAATAGATAAAATGATTGCACTAGGCCTTTTAACAGAGGTTAAATATGACTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10414.90 Da
• Isoelectric Point: 9.0065

>AT201472 WP_110075591.1 NZ_CP074112:c2674861-2674586 [Idiomarina loihiensis]
MNLEQLKKQALKNPEVKAEYDNLEDEFNLIHQLLNMRAQAGLTQEQLAKRMHTSKSNISRLERGNTNPSWATLVKYAQAC
GFELSLMAHKS

Nucleotide

• Length: 276 bp

>AT201472 NZ_CP098446:3722372-3722692 [Proteus mirabilis]
ATGACTGATAAAATGAAAGAAATGCTAAAATTTGCACAAGAAATGGAAGATGCAGGAGTCACTGATGGCAGTACAACTAA
ACGTATTGCCGCCAGAATAAAAGCGAAAGAATTACAAGCTAAGTATAAACATCCTACCCCTATGACAGGTGAAGATATAA
AAAGGATCCGTGAGCAAAATAATATCTCTCAATCTTCCCTTGCTTTGATATTGAATATGACTAAAGAAAGCGTTTCTAAG
TGGGAAAGAAACGAAATTAAGCCCAATGGTGCAGCACTACGACTTCTTCATTTAATAGATAAAAAAATATTATTCATCTA
G