Detailed information of TA system TA201431

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4242178..4242780
• Replicon: chromosome
• Accession: NZ_CP074096
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain 2B_cip

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A603E470
• Locus tag: KFV00_RS20605
• Protein ID: WP_023138876.1
• Coordinates: 4242178..4242489 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KFV00_RS20610
• Protein ID: WP_000362052.1
• Coordinates: 4242490..4242780 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFV00_RS20570 (4237291)	4237291..4237890	+	600	WP_000965695.1	glucose-1-phosphatase	-
KFV00_RS20575 (4237884)	4237884..4238756	+	873	WP_000921423.1	virulence factor BrkB family protein	-
KFV00_RS20580 (4238753)	4238753..4239190	+	438	WP_000560969.1	D-aminoacyl-tRNA deacylase	-
KFV00_RS20585 (4239235)	4239235..4240176	+	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
KFV00_RS20590 (4240191)	4240191..4240637	-	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
KFV00_RS20595 (4240634)	4240634..4240945	-	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
KFV00_RS20600 (4241031)	4241031..4241960	-	930	WP_212634352.1	alpha/beta hydrolase	-
KFV00_RS20605 (4242178)	4242178..4242489	+	312	WP_023138876.1	hypothetical protein	Toxin
KFV00_RS20610 (4242490)	4242490..4242780	+	291	WP_000362052.1	DNA-binding transcriptional regulator	Antitoxin
KFV00_RS20615 (4242827)	4242827..4243756	-	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
KFV00_RS20620 (4243753)	4243753..4244388	-	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
KFV00_RS20625 (4244385)	4244385..4245287	-	903	WP_000331361.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-74)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12356.30 Da
• Isoelectric Point: 9.4460

>T201431 WP_023138876.1 NZ_CP074096:4242178-4242489 [Salmonella enterica subsp. enterica serovar Typhimurium]
MQFIETELFTEDVKKLLDEDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGSRGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T201431 NZ_CP098408:c244650-244354 [Alcaligenes sp. DN25]
ATGATTAAGAGCTTCCGACACAAGGGTCTTCAGGCTTACTTTGAAACCGGCTCCAAATCCGGCATTCAAGCCAAGCATGC
TGTCCGCCTCCAGATTCAGCTAACAGCCCTGCACGCTGCTACTCAAGCGGAAGATATGAACGCCCCCGGATGGAAGCTCC
ACAAGTTATCGGGCAAAAACCCCAAAGGCCAATCTGTACAAGATCACTACACAATATCGGTTAGCGGGAACTGGCGTCTA
ACCTTTTACTTTGAAGGCGAAGACGCTGTTTTGGTGGACTACCAGGACTATCACTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10970.65 Da
• Isoelectric Point: 11.0303

>AT201431 WP_000362052.1 NZ_CP074096:4242490-4242780 [Salmonella enterica subsp. enterica serovar Typhimurium]
MDKVLFERLTQSMSQMNEIIEGTRKPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT201431 NZ_CP098408:c244344-244060 [Alcaligenes sp. DN25]
ATGCGTATGCACAACCCATCCCACCCCGGCCCAGTCTTGCGGGAATACTTGGGCGAACTCAGTGTCACAGAGGCGGCATC
CCGTCTTGGCGTAACGCGTGCAGCCTTATCGCGCATCCTCAATGGTTCTGCCGGTATTTCGGCAGACATGGCCTTACGTC
TGCGCGACGCGCTTGGAACCAGCGCCGAGATGTGGATCAATATGCAGTCCCACTACGATCTGTGGCAGGCAGAACAAAAA
CCAAGGCCCAAAGTGCTGTCCTTCCATGGCCCCGCCCATGCCTGA

Structures

Toxin

• AlphaFold DB: A0A603E470