Detailed information of TA system TA201407

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4242004..4242606
• Replicon: chromosome
• Accession: NZ_CP074094
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain A_cip

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A603E470
• Locus tag: KFU99_RS20610
• Protein ID: WP_023138876.1
• Coordinates: 4242004..4242315 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KFU99_RS20615
• Protein ID: WP_000362052.1
• Coordinates: 4242316..4242606 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU99_RS20575 (4237117)	4237117..4237716	+	600	WP_000965695.1	glucose-1-phosphatase	-
KFU99_RS20580 (4237710)	4237710..4238582	+	873	WP_000921423.1	virulence factor BrkB family protein	-
KFU99_RS20585 (4238579)	4238579..4239016	+	438	WP_000560969.1	D-aminoacyl-tRNA deacylase	-
KFU99_RS20590 (4239061)	4239061..4240002	+	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
KFU99_RS20595 (4240017)	4240017..4240463	-	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
KFU99_RS20600 (4240460)	4240460..4240771	-	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
KFU99_RS20605 (4240857)	4240857..4241786	-	930	WP_001127703.1	alpha/beta hydrolase	-
KFU99_RS20610 (4242004)	4242004..4242315	+	312	WP_023138876.1	hypothetical protein	Toxin
KFU99_RS20615 (4242316)	4242316..4242606	+	291	WP_000362052.1	DNA-binding transcriptional regulator	Antitoxin
KFU99_RS20620 (4242653)	4242653..4243582	-	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
KFU99_RS20625 (4243579)	4243579..4244214	-	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
KFU99_RS20630 (4244211)	4244211..4245113	-	903	WP_000331361.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-74)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12356.30 Da
• Isoelectric Point: 9.4460

>T201407 WP_023138876.1 NZ_CP074094:4242004-4242315 [Salmonella enterica subsp. enterica serovar Typhimurium]
MQFIETELFTEDVKKLLDEDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGSRGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T201407 NZ_CP098352:c5422454-5422071 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10970.65 Da
• Isoelectric Point: 11.0303

>AT201407 WP_000362052.1 NZ_CP074094:4242316-4242606 [Salmonella enterica subsp. enterica serovar Typhimurium]
MDKVLFERLTQSMSQMNEIIEGTRKPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT201407 NZ_CP098352:c5422696-5422454 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A603E470