Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3999393..4000087 Replicon chromosome
Accession NZ_CP074052
Organism Escherichia coli strain PD23

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag KFU85_RS19320 Protein ID WP_001263491.1
Coordinates 3999393..3999791 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag KFU85_RS19325 Protein ID WP_000554755.1
Coordinates 3999794..4000087 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU85_RS19290 (3994655) 3994655..3996112 + 1458 WP_022645227.1 cytosol nonspecific dipeptidase -
KFU85_RS19295 (3996121) 3996121..3996402 + 282 WP_022645226.1 hypothetical protein -
KFU85_RS19300 (3996419) 3996419..3996928 - 510 WP_001361775.1 metal-dependent hydrolase -
KFU85_RS19305 (3996990) 3996990..3997604 - 615 WP_022645225.1 peptide chain release factor H -
KFU85_RS19310 (3997601) 3997601..3998740 - 1140 WP_022645224.1 RNA ligase RtcB family protein -
KFU85_RS19315 (3998931) 3998931..3999383 - 453 WP_023909048.1 GNAT family N-acetyltransferase -
KFU85_RS19320 (3999393) 3999393..3999791 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
KFU85_RS19325 (3999794) 3999794..4000087 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
KFU85_RS19330 (4000139) 4000139..4001194 - 1056 WP_022645222.1 DNA polymerase IV -
KFU85_RS19335 (4001265) 4001265..4002188 - 924 WP_001232555.1 putative lateral flagellar export/assembly protein LafU -
KFU85_RS19340 (4002191) 4002191..4003054 - 864 WP_001169518.1 flagellar motor stator protein MotA -
KFU85_RS19345 (4003067) 4003067..4003783 - 717 WP_088129960.1 FliA/WhiG family RNA polymerase sigma factor -
KFU85_RS19350 (4003803) 4003803..4004270 - 468 WP_000725260.1 flagellar basal body-associated FliL family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T201327 WP_001263491.1 NZ_CP074052:c3999791-3999393 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T201327 NZ_CP098337:c6108-5803 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCTGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT201327 WP_000554755.1 NZ_CP074052:c4000087-3999794 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT201327 NZ_CP098337:c6328-6110 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGTGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References