Detailed information of TA system TA201308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 631930..632729
• Replicon: chromosome
• Accession: NZ_CP074052
• Organism: Escherichia coli strain PD23

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: KFU85_RS03010
• Protein ID: WP_000347273.1
• Coordinates: 631930..632394 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: KFU85_RS03015
• Protein ID: WP_001308975.1
• Coordinates: 632394..632729 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU85_RS02980 (626931)	626931..627365	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
KFU85_RS02985 (627383)	627383..628261	-	879	WP_001298314.1	PTS N-acetylgalactosamine transporter subunit IID	-
KFU85_RS02990 (628251)	628251..629030	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
KFU85_RS02995 (629041)	629041..629514	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
KFU85_RS03000 (629537)	629537..630817	-	1281	WP_023908831.1	tagatose-bisphosphate aldolase subunit KbaZ	-
KFU85_RS03005 (631066)	631066..631875	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
KFU85_RS03010 (631930)	631930..632394	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
KFU85_RS03015 (632394)	632394..632729	-	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
KFU85_RS03020 (632878)	632878..634449	-	1572	WP_001273756.1	galactarate dehydratase	-
KFU85_RS03025 (634824)	634824..636158	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
KFU85_RS03030 (636174)	636174..636944	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T201308 WP_000347273.1 NZ_CP074052:c632394-631930 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T201308 NZ_CP098330:3807524-3807742 [Citrobacter freundii]
ATGTCCGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGTTGCCAGACAATTGATACGCTAGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACCATGAATAAGCTTTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT201308 WP_001308975.1 NZ_CP074052:c632729-632394 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT201308 NZ_CP098330:3807122-3807496 [Citrobacter freundii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACTCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCAACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08