Detailed information of TA system TA201281

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4446629..4447231
• Replicon: chromosome
• Accession: NZ_CP074042
• Organism: Escherichia coli strain PI6

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: KFU63_RS21275
• Protein ID: WP_000897305.1
• Coordinates: 4446920..4447231 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KFU63_RS21270
• Protein ID: WP_000356397.1
• Coordinates: 4446629..4446919 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU63_RS21245 (4442555)	4442555..4443457	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
KFU63_RS21250 (4443454)	4443454..4444089	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
KFU63_RS21255 (4444086)	4444086..4445015	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
KFU63_RS21260 (4445345)	4445345..4445587	-	243	WP_001086388.1	protein YiiF	-
KFU63_RS21265 (4445806)	4445806..4446024	-	219	WP_001295676.1	CopG family transcriptional regulator	-
KFU63_RS21270 (4446629)	4446629..4446919	-	291	WP_000356397.1	NadS family protein	Antitoxin
KFU63_RS21275 (4446920)	4446920..4447231	-	312	WP_000897305.1	hypothetical protein	Toxin
KFU63_RS21280 (4447460)	4447460..4448368	+	909	WP_001162704.1	alpha/beta hydrolase	-
KFU63_RS21285 (4448432)	4448432..4449373	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
KFU63_RS21290 (4449418)	4449418..4449855	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
KFU63_RS21295 (4449852)	4449852..4450724	-	873	WP_000920762.1	virulence factor BrkB family protein	-
KFU63_RS21300 (4450718)	4450718..4451317	-	600	WP_001315111.1	glucose-1-phosphatase	-
KFU63_RS21305 (4451416)	4451416..4452201	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T201281 WP_000897305.1 NZ_CP074042:c4447231-4446920 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T201281 NZ_CP098257:2215921-2216283 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACATACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGTTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT201281 WP_000356397.1 NZ_CP074042:c4446919-4446629 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT201281 NZ_CP098257:2215754-2215924 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7