Detailed information of TA system TA201265

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1837598..1838100
• Replicon: chromosome
• Accession: NZ_CP074042
• Organism: Escherichia coli strain PI6

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: KFU63_RS08795
• Protein ID: WP_000767829.1
• Coordinates: 1837846..1838100 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: KFU63_RS08790
• Protein ID: WP_001259255.1
• Coordinates: 1837598..1837849 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU63_RS08765 (1832776)	1832776..1833843	-	1068	WP_000080141.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
KFU63_RS08770 (1833843)	1833843..1834913	-	1071	WP_032248775.1	histidinol-phosphate transaminase	-
KFU63_RS08775 (1834910)	1834910..1836214	-	1305	WP_001752760.1	histidinol dehydrogenase	-
KFU63_RS08780 (1836220)	1836220..1837119	-	900	WP_000131776.1	ATP phosphoribosyltransferase	-
KFU63_RS08785 (1837265)	1837265..1837315	-	51	WP_001364200.1	his operon leader peptide	-
KFU63_RS08790 (1837598)	1837598..1837849	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
KFU63_RS08795 (1837846)	1837846..1838100	+	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
KFU63_RS08800 (1838183)	1838183..1839007	+	825	WP_000754749.1	SDR family oxidoreductase	-
KFU63_RS08805 (1839053)	1839053..1839982	+	930	WP_000803337.1	LysR substrate-binding domain-containing protein	-
KFU63_RS08810 (1840197)	1840197..1840259	+	63	WP_010723108.1	membrane protein YoeI	-
KFU63_RS08815 (1840249)	1840249..1841607	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
KFU63_RS08820 (1841786)	1841786..1842844	+	1059	WP_000492340.1	thiosulfate utilization transporter TsuA/YeeE	-
KFU63_RS08825 (1842858)	1842858..1843085	+	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T201265 WP_000767829.1 NZ_CP074042:1837846-1838100 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T201265 NZ_CP098253:2215930-2216292 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACATACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGTTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT201265 WP_001259255.1 NZ_CP074042:1837598-1837849 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT201265 NZ_CP098253:2215763-2215933 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0